Document Detail

Glucosamine inhibits epithelial-to-mesenchymal transition and migration of retinal pigment epithelium cells in culture and morphologic changes in a mouse model of proliferative vitreoretinopathy.
MedLine Citation:
PMID:  21457483     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To explore the effect of glucosamine (GlcN) on transforming growth factor (TGF)-β signalling and several processes involved in proliferative vitreoretinopathy (PVR).
METHODS: We evaluated the surface levels of TGF-β receptor and its binding of TGF-β in ARPE-19 cells. Release of cytokines and collagen, and expression of signalling intermediates were quantified. Migration was qualitatively and quantitatively examined. The morphology of cells undergoing PVR in vitro and in a mouse PVR model was observed.
RESULTS: Glucosamine reduced the surface levels of TGF-β receptor and the ability of ARPE-19 cells to bind TGF-β. In ARPE-19 cells, TGF-β1 plus epidermal growth factor (EGF) or TGF-β2 increased the expression of alpha-smooth muscle actin (α-SMA) and decreased the expression of zona occludens protein (ZO-1). Transforming growth factor-(β2) also caused the release of platelet-derived growth factor (PDGF), connective tissue growth factor (CTGF) and type 1 collagen and increased the phosphorylation of SMAD2 and SMAD3. Platelet-derived growth factor and CTGF stimulated cell migration, and TGF-β2 stimulated wound closure, contraction of collagen and changes in cell morphology.
CONCLUSIONS: Treatment with GlcN counteracted all of these effects, and its administration in the mouse model reduced the morphologic appearance of PVR. Glucosamine could inhibit the TGF-β signalling pathway in retinal pigment epithelium cells and several of the downstream events associated with epithelial-mesenchymal transition and PVR.
Chang-Min Liang; Ming-Cheng Tai; Yun-Hsiang Chang; Yi-Hao Chen; Ching-Long Chen; Da-Wen Lu; Jiann-Torng Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-01
Journal Detail:
Title:  Acta ophthalmologica     Volume:  89     ISSN:  1755-3768     ISO Abbreviation:  Acta Ophthalmol     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-02     Completed Date:  2011-12-12     Revised Date:  2014-07-30    
Medline Journal Info:
Nlm Unique ID:  101468102     Medline TA:  Acta Ophthalmol     Country:  England    
Other Details:
Languages:  eng     Pagination:  e505-14     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors. Acta Ophthalmologica © 2011 Acta Ophthalmologica Scandinavica Foundation.
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MeSH Terms
Actins / metabolism
Blotting, Western
Cell Movement / drug effects
Cells, Cultured
Collagen / metabolism
Cytokines / metabolism
Disease Models, Animal*
Enzyme-Linked Immunosorbent Assay
Epithelial-Mesenchymal Transition / drug effects*
Glucosamine / pharmacology*
Membrane Proteins / metabolism
Phosphoproteins / metabolism
Receptors, Transforming Growth Factor beta / metabolism
Retinal Pigment Epithelium / physiology*
Signal Transduction
Transforming Growth Factor beta / metabolism
Vitreoretinopathy, Proliferative / drug therapy*,  metabolism,  pathology
Zonula Occludens-1 Protein
Reg. No./Substance:
0/Acta2 protein, mouse; 0/Actins; 0/Cytokines; 0/Membrane Proteins; 0/Phosphoproteins; 0/Receptors, Transforming Growth Factor beta; 0/TJP1 protein, human; 0/Tjp1 protein, mouse; 0/Transforming Growth Factor beta; 0/Zonula Occludens-1 Protein; 9007-34-5/Collagen; N08U5BOQ1K/Glucosamine

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