| Glucosamine HCl alters production of inflammatory mediators by rat intervertebral disc cells in vitro. | |
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MedLine Citation:
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PMID: 17905323 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND CONTEXT: Studies on cartilage have shown anti-inflammatory effects of glucosamine related to inhibition of inflammatory mediators. Intradiscal injection of glucosamine has been proposed as a treatment for chronic discogenic low back pain. However, there have been no studies of the direct effects of glucosamine on disc cells. PURPOSE: To determine the effects of glucosamine HCl on pro-inflammatory mediator production by intervertebral disc cells. STUDY DESIGN: An in vitro, experimental study of interleukin-1 (IL-1) stimulated rat intervertebral disc cells treated with and without glucosamine HCl. METHODS: Rat annulus and nucleus cells were cultured in alginate beads and exposed to IL-1a (10 ng/mL)+glucosamine HCl (4.5 mg/mL), IL-1 alone, or neither for 4 and 7 days. Cell viability and IL-6, tumor necrosis factor alpha (TNF-alpha), prostaglandin E(2) (PGE(2)), and NO levels in the medium were quantified and compared across treatments. RESULTS: Annulus cells, 7 days: Glucosamine completely inhibited IL-6 and TNF-alpha, increased NO (by 75%), and reduced viability (by 89%) compared with IL-1 alone. Nucleus cells, 7 days: Glucosamine reduced IL-6 (by 89%), PGE(2) (91%), and NO (90%) with no effect to viability. CONCLUSIONS: Glucosamine inhibits inflammatory mediator production by IL-1 stimulated disc cells, but also adversely affects the viability of rat annulus cells. The response is cell-type dependent, illustrated by differences for annulus and nucleus cells. |
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Authors:
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Andrew J L Walsh; Conor W O'neill; Jeffrey C Lotz |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-02 |
Journal Detail:
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Title: The spine journal : official journal of the North American Spine Society Volume: 7 ISSN: 1529-9430 ISO Abbreviation: Spine J Publication Date: 2007 Sep-Oct |
Date Detail:
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Created Date: 2007-10-01 Completed Date: 2008-01-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101130732 Medline TA: Spine J Country: United States |
Other Details:
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Languages: eng Pagination: 601-8 Citation Subset: IM |
Affiliation:
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Department of Orthopaedic Surgery, Orthopaedic Bioengineering Laboratory, University of California, San Francisco, 533 Parnassus Ave., Box 0514, San Francisco, CA 94143-0514, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Survival / drug effects Cells, Cultured Dinoprostone / metabolism Extracellular Matrix / immunology, metabolism Glucosamine / pharmacology* Inflammation Mediators / metabolism* Interleukin-1alpha / pharmacology Interleukin-6 / metabolism Intervertebral Disk / cytology*, drug effects*, immunology Male Nitric Oxide / metabolism Rats Rats, Sprague-Dawley Tumor Necrosis Factor-alpha / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Inflammation Mediators; 0/Interleukin-1alpha; 0/Interleukin-6; 0/Tumor Necrosis Factor-alpha; 10102-43-9/Nitric Oxide; 3416-24-8/Glucosamine; 363-24-6/Dinoprostone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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