| Gluconeogenesis and phosphate reabsorption in isolated lactate- or pyruvate-perfused rat kidneys. | |
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MedLine Citation:
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PMID: 6493157 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent experiments suggest that cytoplasmic free NAD inhibits renal phosphate reabsorption and that gluconeogenesis, by increasing NAD concentration, inhibits phosphate reabsorption. To examine these relationships further we measured phosphate reabsorption by isolated kidneys perfused with either lactate or pyruvate. Lactate perfusion increased the ratio of the cytoplasmic redox couple (glycerol-3-phosphate/dihydroxyacetone phosphate) in snap-frozen kidneys 3-fold relative to the ratio with pyruvate perfusion (p less than 0.001). Inulin clearance and fractional phosphate reabsorption were lower with lactate than with pyruvate perfusion. Phosphate reabsorption decreased throughout the lactate perfusion but was constant for 1 h of pyruvate perfusion. Basal and norepinephrine (NE)-stimulated gluconeogenesis were lower with lactate than with pyruvate perfusion. With lactate and pyruvate perfusions, NE induced equal increases in fractional sodium reabsorption, although only with pyruvate perfusion was there increased phosphate reabsorption. In these experiments, oxidative phosphorylation, not cytoplasmic free NAD concentrations, influenced phosphate reabsorption, and NE-stimulated gluconeogenesis was associated with increased phosphate reabsorption. |
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Authors:
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A D Baines; B D Ross |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Mineral and electrolyte metabolism Volume: 10 ISSN: 0378-0392 ISO Abbreviation: Miner Electrolyte Metab Publication Date: 1984 |
Date Detail:
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Created Date: 1984-11-28 Completed Date: 1984-11-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7802196 Medline TA: Miner Electrolyte Metab Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 286-91 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Animals Cytoplasm / metabolism Dihydroxyacetone Phosphate / analysis Glomerular Filtration Rate / drug effects Gluconeogenesis* / drug effects Glycerophosphates / analysis Inulin / metabolism Kidney / drug effects, metabolism* Lactates / metabolism*, pharmacology Male NAD / metabolism Norepinephrine / pharmacology Perfusion Phosphates / metabolism*, urine Pyruvates / metabolism*, pharmacology Rats Sodium / metabolism, urine |
| Chemical | |
Reg. No./Substance:
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0/Glycerophosphates; 0/Lactates; 0/Phosphates; 0/Pyruvates; 51-41-2/Norepinephrine; 53-84-9/NAD; 57-03-4/alpha-glycerophosphoric acid; 57-04-5/Dihydroxyacetone Phosphate; 7440-23-5/Sodium; 9005-80-5/Inulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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