| Glucocorticosteroids and in vitro effects on chemiluminescence of isolated bovine blood granulocytes. | |
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MedLine Citation:
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PMID: 9754921 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of glucocorticosteroids on respiratory burst of bovine granulocytes were studied in vitro by means of (1) chemiluminescence (luminol-dependent, phorbol 12-myristate 13-acetate (PMA)-stimulated), (2) a cell-free chemiluminescence assay, and (3) a myeloperoxidase assay. Significant effects on cellular chemiluminescence were only observed at the highest, not obtainable in vivo, concentration for all drugs except betamethasone. Prednisolone induced inhibition at therapeutic doses. Also, flumethasone and dexamethasone induced significant inhibition at lower concentrations. In the cell-free assay, all glucocorticosteroids, except betamethasone, inhibited chemiluminescence at high concentrations. None of the glucocorticosteroids tested affected myeloperoxidase activity. The results indicated that the drugs do not affect NADPH-oxidase activity. The adverse effects may be due to scavenging of free oxygen radicals, or to interference with the interaction between luminol and the myeloperoxidase-H2O2-halide system. It can be concluded that most glucocorticosteroids show no adverse effects on the respiratory burst of bovine granulocytes in vitro at therapeutical concentrations. |
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Authors:
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D Hoeben; C Burvenich; A M Massart-Leën |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of pharmacology Volume: 354 ISSN: 0014-2999 ISO Abbreviation: Eur. J. Pharmacol. Publication Date: 1998 Aug |
Date Detail:
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Created Date: 1998-12-07 Completed Date: 1998-12-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1254354 Medline TA: Eur J Pharmacol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 197-203 Citation Subset: IM |
Affiliation:
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Department of Physiology, Biochemistry and Biometrics, University of Ghent, Faculty of Veterinary Medicine, Merelbeke, Belgium. dagmar.hoeben@rug.ac.be |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Cell-Free System / drug effects Chemiluminescent Measurements Dose-Response Relationship, Drug Female Glucocorticoids / pharmacology Neutrophils / drug effects*, physiology Peroxidase / antagonists & inhibitors, metabolism Respiratory Burst / drug effects Steroids / pharmacology* Tetradecanoylphorbol Acetate / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Glucocorticoids; 0/Steroids; 16561-29-8/Tetradecanoylphorbol Acetate; EC 1.11.1.7/Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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