Document Detail


Glucocorticosteroid dependent decrease in the activity of calcineurin in the peripheral blood mononuclear cells of patients with systemic lupus erythematosus.
MedLine Citation:
PMID:  11247869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To compare the activity of calcineurin in the peripheral blood mononuclear cells (PBMC) of 32 patients with systemic lupus erythematosus (SLE) and 35 healthy controls. METHODS: The activity of calcineurin was assayed in the supernatants of sonicated mononuclear cells. RESULTS: There was no significant difference in the calcineurin activity of patients with SLE not taking glucocorticosteroids (GCS) compared with the healthy controls. On the other hand, the activity of calcineurin was reduced in patients with SLE taking GCS, correlating negatively with the dose of GCS. The stimulation of PBMC by phorbol ester and calcium ionophore decreased the calcineurin activity both in patients with SLE and in healthy controls. GCS could also reduce calcineurin activity in the mononuclear cells of healthy subjects in vitro. CONCLUSIONS: In patients with SLE the decrease in the calcineurin activity of PBMC depended on the dose of GCS used for treatment, and it was not a disease specific alteration. The higher the dose of GCS, the greater the inhibition of calcineurin activity. The reduction of calcineurin activity is a new element in the immunosuppressive effects of GCS during the treatment of patients with SLE.
Authors:
S Sipka; K Szucs; S Szántó; I Kovács; G Lakos; E Kiss; P Antal-Szalmás; G Szegedi; P Gergely
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of the rheumatic diseases     Volume:  60     ISSN:  0003-4967     ISO Abbreviation:  Ann. Rheum. Dis.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-03-15     Completed Date:  2001-04-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0372355     Medline TA:  Ann Rheum Dis     Country:  England    
Other Details:
Languages:  eng     Pagination:  380-4     Citation Subset:  IM    
Affiliation:
3rd Department of Internal Medicine, Faculty of Medicine, University of Debrecen, H-4004 Debrecen, Hungary. sipka@iiibel.dote.hu
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Calcineurin / blood,  drug effects*
Case-Control Studies
Cells, Cultured
Dose-Response Relationship, Drug
Female
Glucocorticoids / therapeutic use*
Humans
Ionophores / pharmacology
Leukocytes, Mononuclear / drug effects*,  metabolism
Lupus Erythematosus, Systemic / blood,  drug therapy*
Male
Methylprednisolone / therapeutic use*
Middle Aged
Tetradecanoylphorbol Acetate / pharmacology
Chemical
Reg. No./Substance:
0/Glucocorticoids; 0/Ionophores; 16561-29-8/Tetradecanoylphorbol Acetate; 83-43-2/Methylprednisolone; EC 3.1.3.16/Calcineurin
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