Document Detail


Glucocorticoids decrease cytochrome c oxidase activity of isolated rat kidney mitochondria.
MedLine Citation:
PMID:  9755852     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The importance of mitochondria is rising as a target in pathologic processes such as ischemia. We have investigated the effects of hydrocortisone, prednisolone, dexamethasone and triamcinolone on oxidative phosphorylation, Ca2+ fluxes, swelling and membrane potentials in isolated kidney mitochondria. The measurement of respiration state 3 showed a significant decrease in presence of glucocorticoids whereas the other respiration states were not modified. When mitochondria were uncoupled and either the complexes III and IV or the complex IV were stimulated, the O2 consumption was decreased by glucocorticoids. These results suggest the cytochrome c oxidase is a target of the glucocorticoid effect on the respiratory chain. Indeed, the other mitochondrial functions investigated were unchanged, ruling out a direct effect on Ca2+ fluxes or swelling. A regulation of cytochrome c oxidase activity by glucocorticoids will be of particular interest in pathology involving metabolic insult.
Authors:
N Simon; P Jolliet; C Morin; R Zini; S Urien; J P Tillement
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  435     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-10-05     Completed Date:  1998-10-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  25-8     Citation Subset:  IM    
Affiliation:
Départment de Pharmacologie, Faculté de Médecine de Paris XII, Créteil, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport / drug effects
Calcium / metabolism
Dexamethasone / pharmacology
Electron Transport Complex IV / drug effects*,  metabolism*
Enzyme Activation / drug effects
Glucocorticoids / pharmacology*
Hydrocortisone / pharmacology
Kidney / drug effects,  enzymology*,  physiology
Male
Membrane Potentials / drug effects
Mitochondria / enzymology*,  physiology
Mitochondrial Swelling / drug effects
Oxygen Consumption / drug effects
Prednisolone / pharmacology
Rats
Rats, Wistar
Triamcinolone / pharmacology
Chemical
Reg. No./Substance:
0/Glucocorticoids; 124-94-7/Triamcinolone; 50-02-2/Dexamethasone; 50-23-7/Hydrocortisone; 50-24-8/Prednisolone; 7440-70-2/Calcium; EC 1.9.3.1/Electron Transport Complex IV

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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