| Glucocorticoid receptor distribution in rat testis during postnatal development and effects of dexamethasone on immature peritubular cells in vitro. | |
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MedLine Citation:
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PMID: 10702863 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, the occurrence of the glucocorticoid receptor in the rat testis during early stages of postnatal development and its potential functional significance were investigated. Quantitative analyses of immunohistochemically labelled paraffin sections revealed that the receptor was present during all stages of postnatal development in the nuclei of interstitial cells such as Leydig cells, macrophages and fibroblasts, and endothelial cells of blood vessels. The labelling index increased initially, with maximum levels reached within the second week of postnatal development, and decreased thereafter. Within the seminiferous tubules, the glucocorticoid receptor could be detected in the nuclei of germ cells as well as Sertoli cells, reaching the highest levels in 3-week-old rats, mainly due to immature germ cell staining. In contrast, approximately 50% of the peritubular cell nuclei were stained throughout postnatal development. In vitro experiments on immature and immortalized peritubular cells demonstrated a dose-dependent and significant decrease in proliferation and fibronectin secretion after administration of dexamethasone. The data of this study suggest that glucocorticoids have a consistently repressive effect on peritubular cells throughout postnatal development. In summary, labelling of germ cells, especially in immature rats, might indicate an inhibition of spermatogenesis by corticosteroids. |
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Authors:
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M A Weber; S Groos; U Höpfl; M Spielmann; G Aumüller; L Konrad |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Andrologia Volume: 32 ISSN: 0303-4569 ISO Abbreviation: Andrologia Publication Date: 2000 Jan |
Date Detail:
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Created Date: 2000-04-11 Completed Date: 2000-04-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0423506 Medline TA: Andrologia Country: GERMANY |
Other Details:
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Languages: eng Pagination: 23-30 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Cell Biology, University of Marburg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division / drug effects Cell Line Dexamethasone / pharmacology Female Fibronectins / metabolism Immunohistochemistry Male Rats Rats, Wistar Receptors, Glucocorticoid / metabolism* Spermatogenesis / drug effects Testis / drug effects, growth & development*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fibronectins; 0/Receptors, Glucocorticoid; 50-02-2/Dexamethasone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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