Document Detail

Glucocorticoid- and nerve growth factor-induced changes in chromogranin A expression define two different neuronal phenotypes in PC12 cells.
MedLine Citation:
PMID:  3185567     Owner:  NLM     Status:  MEDLINE    
The regulation of chromogranin A mRNA was examined in PC12 cells after treatment with nerve growth factor, dexamethasone, or a combination of the two agents. PC12 cells have low levels of chromogranin A mRNA, and this does not change upon treatment with nerve growth factor. Dexamethasone treatment of these cells results in a 4-fold increase in the amount of chromogranin A mRNA. The dexamethasone-stimulated increase in chromogranin A mRNA is not apparent until at least 16 h after the addition of the drug and is maintained only with continuous culture in the presence of the drug. Dexamethasone and nerve growth factor together increase chromogranin A mRNA to the level seen with dexamethasone alone. Immunohistochemistry shows a similar pattern of protein accumulation within individual cells. Chromogranin B mRNA levels are unaltered by any of the drug treatments described. Treatment with dexamethasone plus NGF seems to be required for full expression of the adrenergic, neuronal phenotype in PC12 cells. Measurement of chromogranin A mRNA provides more specific delineation of neural differentiation and how it is influenced by hormones and growth factors.
D M Rausch; A L Iacangelo; L E Eiden
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular endocrinology (Baltimore, Md.)     Volume:  2     ISSN:  0888-8809     ISO Abbreviation:  Mol. Endocrinol.     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1988-12-20     Completed Date:  1988-12-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8801431     Medline TA:  Mol Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  921-7     Citation Subset:  IM    
Unit on Molecular and Cellular Neurobiology, National Institute of Mental Health, Bethesda, Maryland 20892.
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MeSH Terms
Adrenal Gland Neoplasms / genetics
Chromogranin A
Chromogranins / genetics*
Dexamethasone / physiology*
Glucocorticoids / physiology*
Nerve Growth Factors / physiology*
Nerve Tissue Proteins / genetics*
Neurons / physiology*
Pheochromocytoma / genetics
RNA, Messenger / biosynthesis
Tumor Cells, Cultured
Reg. No./Substance:
0/Chromogranin A; 0/Chromogranins; 0/Glucocorticoids; 0/Nerve Growth Factors; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/chromogranin A, rat; 50-02-2/Dexamethasone

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