Document Detail


Glucans exhibit weak antioxidant activity, but stimulate macrophage free radical activity.
MedLine Citation:
PMID:  11182295     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polymeric carbohydrates have been reported to modulate inflammatory responses in vitro and in vivo. Previous reports suggest that certain carbohydrate polymers, such as (1-->3)-beta-D-glucans, may possess free radical scavenging activity. If glucans are free radical scavengers then it might explain, in part, the ability of these ligands to modulate inflammatory responses. The present study examined the free radical scavenging activity of a variety of carbohydrate polymers and the effect of the polymers on free radical levels in a murine macrophage cell line. All of the carbohydrates exhibited concentration dependent antioxidant effects (EC(50) range = 807 to 43 microg/ml). However, the antioxidant activity for the carbohydrates was modest in comparison with PDTC (EC(50) = 0.13 microg/ml) and the carbohydrate concentration required for antioxidant activity was high (x EC(50) = 283 microg/ml). The antioxidant ability of the polymers was greater (p < .05) than their monosaccharide constituents, i.e., dextrose EC(50) = 807 vs. glucan sulfate EC(50) = 43 microg/ml. Coincubation of glucans with murine J774a.1 cells increased free radical levels when compared to controls. Therefore, the weak free radical scavenging activity of glucan polymers cannot explain their modulatory effect on inflammatory responses in tissue culture and/or disease models of inflammation.
Authors:
E Tsiapali; S Whaley; J Kalbfleisch; H E Ensley; I W Browder; D L Williams
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Free radical biology & medicine     Volume:  30     ISSN:  0891-5849     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-06-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  393-402     Citation Subset:  IM    
Affiliation:
Department of Surgery, East Tennessee State University, Johnson City, TN 37614-0575, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / metabolism*
Cell Line
Flow Cytometry
Free Radicals / metabolism*
Glucans / pharmacology*
Macrophages / drug effects*
Mice
Spectrometry, Fluorescence
Grant Support
ID/Acronym/Agency:
GM53522/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Free Radicals; 0/Glucans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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