Document Detail

Glu298Asp polymorphism influences the beneficial effects of fish oil fatty acids on postprandial vascular function.
MedLine Citation:
PMID:  22847178     Owner:  NLM     Status:  MEDLINE    
Our objective was to determine whether the endothelial nitric oxide synthase (eNOS) Glu298Asp polymorphism influences vascular response to raised NEFA enriched with saturated fatty acids (SFA) or long-chain (LC) n-3 polyunsaturated fatty acids (PUFA). Subjects were prospectively recruited for genotype (Glu298, n = 30 and Asp298, n = 29; balanced for age and gender) consumed SFA on two occasions, with and without the substitution of 0.07 g fat/kg body weight with LC n-3 PUFA, and with heparin infusion to elevate NEFA. Endothelial function was measured before and after NEFA elevation (240 min), with blood samples taken every 30 min. Flow-mediated dilation (FMD) decreased following SFA alone and increased following SFA+LC n-3 PUFA. There were 2-fold differences in the change in FMD response to the different fat loads between the Asp298 and Glu298 genotypes (P = 0.002) and between genders (P < 0.02). Sodium nitroprusside-induced reactivity, measured by laser Doppler imaging with iontophoresis, was significantly greater with SFA+LC n-3 PUFA in all female subjects (P < 0.001) but not in males. Elevated NEFA influences both endothelial-dependent and endothelial-independent vasodilation during the postprandial phase. Effects of fat composition appear to be genotype and gender dependent, with the greatest difference in vasodilatory response to the two fat loads seen in the Asp298 females.
Abby K Thompson; Katie J Newens; Kim G Jackson; John Wright; Christine M Williams
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't     Date:  2012-07-30
Journal Detail:
Title:  Journal of lipid research     Volume:  53     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-07     Completed Date:  2013-01-29     Revised Date:  2013-10-10    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2205-13     Citation Subset:  IM    
Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading, RG6 6AP, UK.
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MeSH Terms
Cross-Over Studies
Endothelium, Vascular / drug effects,  physiology
Fatty Acids / administration & dosage*,  chemistry
Fatty Acids, Nonesterified / metabolism*
Fish Oils / pharmacology*
Middle Aged
Nitric Oxide Synthase Type III / genetics*,  metabolism
Polymorphism, Single Nucleotide*
Postprandial Period
Prospective Studies
Single-Blind Method
Vasodilation / drug effects*,  genetics
Grant Support
BB/E0221816/1//Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Fish Oils; EC Oxide Synthase Type III

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