| Glomerular anionic charge in congenital nephrotic syndrome of the Finnish type. | |
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MedLine Citation:
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PMID: 7591846 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Decrease of the anionic charge of the glomerular basement membrane and especially the reduced amount of heparan sulphate proteoglycan in the lamina rara externa has been suggested to be the basic pathogenetic defect in congenital nephrotic syndrome. In the present study the anionic charge of glomeruli was examined in the congenital nephrotic syndrome of the Finnish type and in controls using cationic stains (polyethyleneimine, Ruthenium Red) in electron microscopy. Chondroitinase and heparinase treatments were used to characterize further the anionic elements detected. Scanning electron microscopy (SEM) was used in addition to transmission electron microscopy (TEM) to examine the tridimensional structure and secondary changes of podocytes in this syndrome. The number (mean +/- SD) of polyethyleneimine granules per 1 micron length of lamina rara externa of the glomerular basement membrane was 24.9 +/- 4.5 in control and 23.2 +/- 4.3 [corrected] in congenital nephrotic syndrome subjects. The Ruthenium Red staining pattern was closely similar in syndrome and control kidneys. The granules evident after staining with either cationic stain were seen after chondroitinase but not after heparinase treatment in control as well as in syndrome patient kidney samples. No denuded areas of basement membrane in 42 glomeruli from four syndrome patients were found in SEM. In conclusion, the amount of anionic sites in the lamina rara externa as detected by either cationic stain was comparable to controls. These results do not support the hypothesis of decreased anionic sites in the lamina rara externa of the glomerular basement membrane in congenital nephrotic syndrome of the Finnish type. |
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Authors:
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P Ljungberg; J Rapola; C Holmberg; H Holthöfer; H Jalanko |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Histochemical journal Volume: 27 ISSN: 0018-2214 ISO Abbreviation: Histochem. J. Publication Date: 1995 Jul |
Date Detail:
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Created Date: 1995-11-30 Completed Date: 1995-11-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0163161 Medline TA: Histochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 536-46 Citation Subset: IM |
Affiliation:
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Department of Bacteriology and Immunology, University of Helsinki, Finland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anions
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analysis* Basement Membrane / metabolism, ultrastructure Child, Preschool Humans Infant Kidney Glomerulus / metabolism*, ultrastructure Microscopy, Electron Microscopy, Electron, Scanning Nephrotic Syndrome / congenital, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Anions |
| Comments/Corrections | |
Erratum In:
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Histochem J 1995 Sep;27(9):724 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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