| Glomerular expression of kidney injury molecule-1 and podocytopenia in diabetic glomerulopathy. | |
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MedLine Citation:
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PMID: 21822010 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/AIMS: Studies have shown that kidney injury molecule-1 (KIM-1) is upregulated in damaged renal proximal tubules. In this study, we examined KIM-1 expression in glomerular epithelial cells in diabetic glomerulopathy. METHODS: Renal histology, immunostaining and Western blot for protein level, and real-time PCR for mRNA expression of KIM-1 and podocyte markers were evaluated in untreated or losartan-treated Zucker lean (Fa/+) and Zucker diabetic fatty (Fa/Fa) rats. RESULTS: The diabetic rats showed an increased glomerular expression of KIM-1. KIM-1 staining was localized primarily in the hyperplastic parietal epithelium of Bowman's capsule in the early stages of diabetes with subsequent increase in KIM-1-positive cells in the glomerular tuft in the more advanced stages. The increase in glomerular KIM-1 was associated with a decrease in podocytes in Fa/Fa rats. Antiproteinuric treatment with losartan attenuated podocytopenia and decreased renal expression of KIM-1 in treated diabetic rats. In an in vitro study, albumin overload increased KIM-1 protein in the primary cultures of rat glomerular epithelial cells. CONCLUSION: These results show that glomerular KIM-1 expression was increased, in proportion to the extent of proteinuria and podocytopenia in the diabetic animals, supporting that KIM-1 could be used as a potential biomarker for glomerular injury in proteinuric kidney disease. |
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Authors:
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Xueying Zhao; Yuanyuan Zhang; Lingyun Li; David Mann; John D Imig; Nerimiah Emmett; Gary Gibbons; Li-Ming Jin |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-08-04 |
Journal Detail:
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Title: American journal of nephrology Volume: 34 ISSN: 1421-9670 ISO Abbreviation: Am. J. Nephrol. Publication Date: 2011 |
Date Detail:
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Created Date: 2011-09-16 Completed Date: 2012-01-25 Revised Date: 2012-04-30 |
Medline Journal Info:
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Nlm Unique ID: 8109361 Medline TA: Am J Nephrol Country: Switzerland |
Other Details:
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Languages: eng Pagination: 268-80 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 S. Karger AG, Basel. |
Affiliation:
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Department of Physiology, Morehouse School of Medicine, Atlanta, GA 30310, USA. xzhao@msm.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion Molecules / biosynthesis* Cells, Cultured Diabetic Nephropathies / metabolism* Epithelial Cells / metabolism* Kidney Glomerulus / cytology* Male Podocytes* Rats Rats, Zucker |
| Grant Support | |
ID/Acronym/Agency:
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G12-RR03034/RR/NCRR NIH HHS; HL59699/HL/NHLBI NIH HHS; R01 HL059699/HL/NHLBI NIH HHS; U54RR026137/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cell Adhesion Molecules; 0/Havcr1protein, rat |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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