Document Detail


Glomerular CD34 expression in short- and long-term diabetes.
MedLine Citation:
PMID:  18319274     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aging and diabetes are associated with exacerbated expression of adhesion molecules. Given their importance in endothelial dysfunction and their possible involvement in the alteration of glomerular permeability occurring in diabetes, we have evaluated expression of the sialomucin-type adhesion molecule CD34 in renal glomerular cells of normal and diabetic animals at two different ages by colloidal gold immunocytochemistry and immunoblotting. CD34 labeling was mostly assigned to the plasma membranes of glomerular endothelium and mesangial processes. Podocyte membranes were also labeled, but to a lesser degree. Short- and long-term diabetes triggers a substantial increase in immunogold labeling for CD34 in renal tissues compared with young normoglycemic animals. However, the level of labeling in old diabetic and healthy control rats is similar, suggesting that the effect of diabetes and aging on CD34 expression is similar but not synergistic. Western blotting of isolated glomerular fractions corroborated immunocytochemical results. Increased expression of CD34 may reflect its involvement in the pathogenesis of glomerular alterations related to age and diabetes. Alterations present in early diabetes, resembling those occurring with age, strengthen the concept that diabetes is an accelerated form of aging.
Authors:
Luz Marina Acevedo; Irene Londono; Malika Oubaha; Lucian Ghitescu; Moise Bendayan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-03
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  56     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-19     Completed Date:  2008-06-05     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  605-14     Citation Subset:  IM    
Affiliation:
Department of Pathology and Cell Biology, Université de Montréal, Montréal QC H3T 1J4, Canada.
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MeSH Terms
Descriptor/Qualifier:
Aging
Amino Acid Sequence
Animals
Antigens, CD34 / biosynthesis*
Blotting, Western
Diabetes Mellitus, Experimental / metabolism*
Hyperglycemia / metabolism
Immunohistochemistry
Kidney Glomerulus / blood supply,  metabolism*
Male
Molecular Sequence Data
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Antigens, CD34
Comments/Corrections

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