| Globosal basal cells are identified as proliferating cells in mouse olfactory epithelium. | |
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MedLine Citation:
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PMID: 11201809 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although anti-bromodeoxyuridine (BrdU) antibodies are used for detecting proliferating cells, they may stain proliferating cells in phases following the S-phase, except for G1. Anti-Ki67 antibodies are widely used for detecting proliferating cells in all phases of the cell cycle (G1-, S-, G2-, and M-phase), but not in resting cells (G0-phase). Anti-cyclin D1 antibodies are used to detect proliferating cells in the G1-phase. The present study investigated the olfactory epithelium of mice by double immunostaining using antibodies to BrdU, Ki67. cyclin D1, and cytokeratin 14 (CK14). The cells positive to the anti-BrdU antibody, the anti-Ki67 antibody, and the anti-cyclin D1 antibody differed from the cells positive to the anti-CK14 antibody. Thus, we confirmed that the proliferating cells in all the phases of the cell cycle, including the G1-phase, were globosal basal cells, which were the precursors of the olfactory cells. |
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Authors:
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Y Ohta; K Ichimura |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Annals of otology, rhinology, and laryngology Volume: 110 ISSN: 0003-4894 ISO Abbreviation: Ann. Otol. Rhinol. Laryngol. Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-01-29 Completed Date: 2001-02-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0407300 Medline TA: Ann Otol Rhinol Laryngol Country: United States |
Other Details:
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Languages: eng Pagination: 53-5 Citation Subset: AIM; IM |
Affiliation:
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Department of Otolaryngology-Head and Neck Surgery, Jichi Medical School, Minamikawachi, Tochigi, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / diagnostic use Bromodeoxyuridine / analysis Cell Cycle Cell Division Cyclin D1 / analysis Immunohistochemistry Keratins / analysis Ki-67 Antigen / analysis Mice Olfactory Mucosa / cytology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Ki-67 Antigen; 136601-57-5/Cyclin D1; 59-14-3/Bromodeoxyuridine; 68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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