Document Detail

Globosal basal cells are identified as proliferating cells in mouse olfactory epithelium.
MedLine Citation:
PMID:  11201809     Owner:  NLM     Status:  MEDLINE    
Although anti-bromodeoxyuridine (BrdU) antibodies are used for detecting proliferating cells, they may stain proliferating cells in phases following the S-phase, except for G1. Anti-Ki67 antibodies are widely used for detecting proliferating cells in all phases of the cell cycle (G1-, S-, G2-, and M-phase), but not in resting cells (G0-phase). Anti-cyclin D1 antibodies are used to detect proliferating cells in the G1-phase. The present study investigated the olfactory epithelium of mice by double immunostaining using antibodies to BrdU, Ki67. cyclin D1, and cytokeratin 14 (CK14). The cells positive to the anti-BrdU antibody, the anti-Ki67 antibody, and the anti-cyclin D1 antibody differed from the cells positive to the anti-CK14 antibody. Thus, we confirmed that the proliferating cells in all the phases of the cell cycle, including the G1-phase, were globosal basal cells, which were the precursors of the olfactory cells.
Y Ohta; K Ichimura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Annals of otology, rhinology, and laryngology     Volume:  110     ISSN:  0003-4894     ISO Abbreviation:  Ann. Otol. Rhinol. Laryngol.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-01-29     Completed Date:  2001-02-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0407300     Medline TA:  Ann Otol Rhinol Laryngol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  53-5     Citation Subset:  AIM; IM    
Department of Otolaryngology-Head and Neck Surgery, Jichi Medical School, Minamikawachi, Tochigi, Japan.
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MeSH Terms
Antibodies / diagnostic use
Bromodeoxyuridine / analysis
Cell Cycle
Cell Division
Cyclin D1 / analysis
Keratins / analysis
Ki-67 Antigen / analysis
Olfactory Mucosa / cytology*
Reg. No./Substance:
0/Antibodies; 0/Ki-67 Antigen; 136601-57-5/Cyclin D1; 59-14-3/Bromodeoxyuridine; 68238-35-7/Keratins

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