Document Detail


Global metabolomics and targeted steroid profiling reveal that rifampin, a strong human PXR activator, alters endogenous urinary steroid markers.
MedLine Citation:
PMID:  23320515     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Activation of the pregnane X receptor (PXR) alters the expression of metabolic enzymes and transporters involved in the metabolism of xenobiotics and endobiotics. To identify endogenous biomarkers of PXR activation in humans, rifampin, a strong PXR activator, was administered to 12 healthy male subjects, and their urine was analyzed before and after rifampin administration. Ultra-performance liquid chromatography time-of-flight mass spectrometry (UPLC/QTof-MS)-based global metabolomics and gas chromatography-mass spectrometry (GC-MS)-based profiling of 75 steroids were used to screen the urine samples. Global metabolomics revealed that hydroxytestosterone sulfate and glycochenodeoxycholate sulfate levels were significantly increased and that androsterone sulfate, dehydroepiandrosterone (DHEA) sulfate, and p-cresol sulfate levels were significantly decreased following rifampin administration compared with controls. Urinary steroid profiling showed that 16α-OH-androstenedione (16α-OH-A-dione), 16α-OH-DHEA, 7α-DHEA, 7β-DHEA, and 11β-OH-A-dione levels were increased, whereas DHEA, androsterone, etiocholanolone, estrone, β-cortolone, and allo-tetrahydrocortisone levels were decreased in the rifampin group. The analysis of the metabolic pathway and the metabolic ratio of steroids enabled the estimation of the induction of CYP1A/3A/7B/11B/2C and the inhibition of CYP17A/19A in response to PXR activation. These human urinary biomarkers may be useful for predicting the extent of PXR activation, monitoring the activity of DMEs, and anticipating drug-drug interactions in patients administered PXR-activating drugs.
Authors:
Bora Kim; Ju-Yeon Moon; Man-Ho Choi; Hyang Hee Yang; Seunghwan Lee; Kyoung Soo Lim; Seo-Hyun Yoon; Kyung-Sang Yu; In-Jin Jang; Joo-Youn Cho
Related Documents :
12617265 - A novel and rapid assay for the detection of neutralizing antibodies against interferon...
23720435 - Efficient purification of high-purity compounds from the stem of lonicera japonica thun...
22312035 - Determination of aflatoxin m1 in milk by high-performance liquid chromatography in mash...
21823235 - Determination of darusentan enantiomers in rat plasma by enantioselective liquid chroma...
20830935 - Pyrolysis-gas chromatography/mass spectrometry identification of distinctive structures...
22659575 - Breath analysis science at pittcon 2012, orlando, florida.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-16
Journal Detail:
Title:  Journal of proteome research     Volume:  -     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Isolated renal metastasis from squamous cell lung cancer.
Next Document:  Air ions and mood outcomes: a review and meta-analysis.