Document Detail

Global metabolomic responses of <i>Escherichia coli</i> HB101 to heat stress.
MedLine Citation:
PMID:  22369756     Owner:  NLM     Status:  Publisher    
Microbial metabolomic analysis is essential for understanding heat stress responses of microorganisms. To understand the comprehensive metabolic responses of <i>Escherichia coli</i> to continuous heat stress, we characterized the metabolomic variations induced by heat stress using NMR spectroscopy in combination with multivariate data analysis. We found 15 amino acids, 10 nucleotides, 9 aliphatic organic acids, 7 amines, glucose and its derivative glucosylglyceric acid, and methanol in the <i>E. coli extracts</i>. Glucosylglyceric acid was reported for the first time in <i>E. coli</i>. We found that heat was an important factor influencing the metabolic state and growth process, mainly via suppressing energy associated metabolism, reducing nucleotide biosynthesis, altering amino acid metabolism and promoting osmotic regulation. Moreover, metabolic perturbation was aggravated during heat stress. However, a sign of recovery to control levels was observed after the removal of heat stress. These findings enhanced our understanding of the metabolic responses of <i>E. coli</i> to heat stress and demonstrated the effectiveness of the NMR-based metabolomics approach to study such a complex system.
Yangfang Ye; Limin Zhang; Fuhua Hao; Jingtao Zhang; Yulan Wang; Huiru Tang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-28
Journal Detail:
Title:  Journal of proteome research     Volume:  -     ISSN:  1535-3907     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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