Document Detail


Global metabolomic responses of <i>Escherichia coli</i> HB101 to heat stress.
MedLine Citation:
PMID:  22369756     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Microbial metabolomic analysis is essential for understanding heat stress responses of microorganisms. To understand the comprehensive metabolic responses of <i>Escherichia coli</i> to continuous heat stress, we characterized the metabolomic variations induced by heat stress using NMR spectroscopy in combination with multivariate data analysis. We found 15 amino acids, 10 nucleotides, 9 aliphatic organic acids, 7 amines, glucose and its derivative glucosylglyceric acid, and methanol in the <i>E. coli extracts</i>. Glucosylglyceric acid was reported for the first time in <i>E. coli</i>. We found that heat was an important factor influencing the metabolic state and growth process, mainly via suppressing energy associated metabolism, reducing nucleotide biosynthesis, altering amino acid metabolism and promoting osmotic regulation. Moreover, metabolic perturbation was aggravated during heat stress. However, a sign of recovery to control levels was observed after the removal of heat stress. These findings enhanced our understanding of the metabolic responses of <i>E. coli</i> to heat stress and demonstrated the effectiveness of the NMR-based metabolomics approach to study such a complex system.
Authors:
Yangfang Ye; Limin Zhang; Fuhua Hao; Jingtao Zhang; Yulan Wang; Huiru Tang
Related Documents :
844876 - Different n-terminal amino acids in the mn-glycoprotein from mm and nn erythrocytes.
6300026 - The application of nucleic acid chemistry to studies on the functional organization of ...
24240426 - Somatic embryogenesis in sitka spruce (picea sitchensis (bong.) carr.).
467856 - Gel electrophoretic analysis of histones in lens epithelium, lens fiber, liver, brain, ...
3880016 - Effect of ascorbate on red blood cell lipid peroxidation.
9760176 - Characterization of an acetyl-11-keto-beta-boswellic acid and arachidonate-binding regu...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-28
Journal Detail:
Title:  Journal of proteome research     Volume:  -     ISSN:  1535-3907     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Reciprocal effects of NNK and SLURP-1 on oncogene expression in target epithelial cells.
Next Document:  Delayed administration of VEGF rescues spinal motor neurons from death with a short effective time f...