Document Detail

Global gene expression analyses of mouse fibroblast L929 cells exposed to IC50 MMA by DNA microarray and confirmation of four detoxification genes' expression by real-time PCR.
MedLine Citation:
PMID:  16916219     Owner:  NLM     Status:  MEDLINE    
Methyl methacrylate (MMA) is the main component of methyl methacrylic resin, which is widely used in dentistry. Previous studies have investigated whether MMA has any adverse effects on growth and gene expression in mouse fibroblast L929 cells. The present study was designed to further understand the effects of MMA by focusing on cDNA microarray data after L929 cells were exposed to MMA. MMA was found to inhibit cell growth and induce detoxification response genes in L929 cells. One of the most highly up-regulated genes was glutathione S-transferase, alpha 1 (Ya) (Gsta1), which has recently been shown to participate in Nrf2 regulation and is considered to be related to detoxification response. Molecular biological data obtained in the present study may therefore provide useful insights into the effects of MMA on living tissue.
Atsuko Ishikawa; Satoshi Jinno; Tomoo Suzuki; Tatsuhide Hayashi; Tatsushi Kawai; Tatsuya Mizuno; Takashi Mori; Masami Hattori
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Dental materials journal     Volume:  25     ISSN:  0287-4547     ISO Abbreviation:  Dent Mater J     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-08-18     Completed Date:  2006-10-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8309299     Medline TA:  Dent Mater J     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  205-13     Citation Subset:  D    
Second Department of Prosthodontics, School of Dentistry, Aichi-gakuin University, 2-11 Suemori-dori, Chikusaku, Nagoyashi, Aichi-ken 464-8651, Japan.
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MeSH Terms
Cell Enlargement / drug effects
Cell Survival / drug effects
Dental Materials / toxicity*
Enzyme Activation
Fibroblasts / drug effects
Gene Expression Profiling
Glutathione Transferase / biosynthesis,  genetics
Inhibitory Concentration 50
Isoenzymes / biosynthesis,  genetics
L Cells (Cell Line)
Metabolic Detoxication, Drug / genetics*
Methylmethacrylate / toxicity*
NF-E2-Related Factor 2 / metabolism
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Reg. No./Substance:
0/Dental Materials; 0/Isoenzymes; 0/NF-E2-Related Factor 2; 80-62-6/Methylmethacrylate; EC Transferase; EC S-transferase alpha

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