| Global changes in phospholipids identified by MALDI MS in rats with focal cerebral ischemia. | |
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MedLine Citation:
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PMID: 22750654 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Neuronal membrane phospholipids are highly affected by oxidative stress caused by ischemic injury. Thus, it is necessary to identify key lipid components that show changes during ischemia to develop an effective approach to prevent brain damage from ischemic injury. The recent development of MALDI imaging MS (MALDI IMS) makes it possible to identify phospholipids that change between damaged and normal regions directly from tissues. In this study, we conducted IMS on rat brains damaged by ischemic injury and detected various phospholipids that showed unique distributions between normal and damaged areas of the brain. Among them, we confirmed changes in phospholipids such as lysophosphatidylcholine, phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin by MALDI IMS followed by MS/MS analysis. These lipids were present in high concentrations in the brain and are important for maintenance of cellular structure as well as production of second messengers for cellular signal transduction. Our results emphasize the identification of phospholipid markers for ischemic injury and successfully identified several distinctly located phospholipids in ischemic brain tissue. |
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Authors:
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Selina Rahman Shanta; Chang Soon Choi; Jeong Hwa Lee; Chan Young Shin; Young Jun Kim; Kyun-Hwan Kim; Kwang Pyo Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-06-29 |
Journal Detail:
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Title: Journal of lipid research Volume: 53 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-08-08 Completed Date: 2012-12-06 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 1823-31 Citation Subset: IM |
Affiliation:
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Department of Molecular Biotechnology, WCU Program, Konkuk University, Seoul 143-701, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / metabolism Brain Ischemia / metabolism* Cerebral Infarction / metabolism Down-Regulation Male Molecular Imaging Molecular Weight Phospholipids / chemistry, metabolism* Potassium / metabolism Principal Component Analysis Rats Rats, Sprague-Dawley Sodium / metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Phospholipids; 7440-09-7/Potassium; 7440-23-5/Sodium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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