| Global assessment of cross-hybridization for oligonucleotide arrays. | |
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MedLine Citation:
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PMID: 16741244 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cross-hybridization is the tendency for chains of nucleic acids to bind to other chains of nucleic acids that have similar but not identical sequences. This has the potential to make the interpretation of microarray experiments difficult since intensity at a spot on the array does not simply depend on the quantity of target in the sample. We propose a method for evaluating the extent of cross-hybridization in oligonucleotide arrays for data arising from a typical microarray experiment. We show that the level of cross-hybridization can be quite substantial. We argue that this makes the interpretation of the difference calls provided by MAS 5.0 difficult. |
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Authors:
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Cavan Reilly; Arvind Raghavan; Paul Bohjanen |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of biomolecular techniques : JBT Volume: 17 ISSN: 1524-0215 ISO Abbreviation: J Biomol Tech Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-06-02 Completed Date: 2006-08-14 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 100888641 Medline TA: J Biomol Tech Country: United States |
Other Details:
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Languages: eng Pagination: 163-72 Citation Subset: IM |
Affiliation:
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Division of Biostatistics, University of Minnesota, A448 Mayo Bldg., MMC 303, 420 Delaware St. SE, Minneapolis, MN 55455-0378, USA. cavanr@biostat.umn.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD28
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chemistry Antigens, CD3 / chemistry Computational Biology Data Interpretation, Statistical Gene Expression Regulation Genomics / methods* Humans Lymphocytes / metabolism Models, Statistical Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis / methods* Oligonucleotide Probes Proteomics / methods* Reproducibility of Results Software |
| Grant Support | |
ID/Acronym/Agency:
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1P30-CA79458-01/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD28; 0/Antigens, CD3; 0/Oligonucleotide Probes |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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