| Global analysis of serine-threonine protein kinase genes in Neurospora crassa. | |
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MedLine Citation:
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PMID: 21965514 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Serine/threonine (S/T) protein kinases are crucial components of diverse signaling pathways in eukaryotes, including the model filamentous fungus Neurospora crassa. In order to assess the importance of S/T kinases to Neurospora biology, we embarked on a global analysis of 86 S/T kinase genes in Neurospora. We were able to isolate viable mutants for 77 of the 86 kinase genes. Of these, 57% exhibited at least one growth or developmental phenotype, with a relatively large fraction (40%) possessing a defect in more than one trait. S/T kinase knockouts were subjected to chemical screening using a panel of eight chemical treatments, with 25 mutants exhibiting sensitivity or resistance to at least one chemical. This brought the total percentage of S/T mutants with phenotypes in our study to 71%. Mutants lacking apg-1, an S/T kinase required for autophagy in other organisms, possessed the greatest number of phenotypes, with defects in asexual and sexual growth and development and in altered sensitivity to five chemical treatments. We showed that NCU02245/stk-19 is required for chemotropic interactions between female and male cells during mating. Finally, we demonstrated allelism between the S/T kinase gene NCU00406 and velvet (vel), encoding a p21-activated protein kinase (PAK) gene important for asexual and sexual growth and development in Neurospora. |
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Authors:
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Gyungsoon Park; Jacqueline A Servin; Gloria E Turner; Lorena Altamirano; Hildur V Colot; Patrick Collopy; Liubov Litvinkova; Liande Li; Carol A Jones; Fitz-Gerald Diala; Jay C Dunlap; Katherine A Borkovich |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2011-09-30 |
Journal Detail:
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Title: Eukaryotic cell Volume: 10 ISSN: 1535-9786 ISO Abbreviation: Eukaryotic Cell Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-02 Completed Date: 2012-05-01 Revised Date: 2012-05-01 |
Medline Journal Info:
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Nlm Unique ID: 101130731 Medline TA: Eukaryot Cell Country: United States |
Other Details:
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Languages: eng Pagination: 1553-64 Citation Subset: IM |
Affiliation:
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Department of Plant Pathology and Microbiology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Fungal Proteins / chemistry, genetics*, metabolism Gene Expression Regulation, Fungal Gene Knockout Techniques Genes, Fungal* Mutation Neurospora crassa / enzymology*, genetics*, physiology Protein-Serine-Threonine Kinases / genetics*, metabolism* Signal Transduction p21-Activated Kinases / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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5T34GM062756/GM/NIGMS NIH HHS; P01 GM068087/GM/NIGMS NIH HHS; R01 GM08336/GM/NIGMS NIH HHS; R01 GM086565/GM/NIGMS NIH HHS; R01 GM086565-04/GM/NIGMS NIH HHS; R01 GM34985/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/p21-Activated Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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