Document Detail


Global transcriptome changes underlying colony growth in the opportunistic human pathogen Aspergillus fumigatus.
MedLine Citation:
PMID:  21724936     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aspergillus fumigatus is the most common and deadly pulmonary fungal infection worldwide. In the lung, the fungus usually forms a dense colony of filaments embedded in a polymeric extracellular matrix. To identify candidate genes involved in this biofilm (BF) growth, we used RNA-Seq to compare the transcriptomes of BF and liquid plankton (PL) growth. Sequencing and mapping of tens of millions sequence reads against the A. fumigatus transcriptome identified 3,728 differentially regulated genes in the two conditions. Although many of these genes, including the ones coding for transcription factors, stress response, the ribosome, and the translation machinery, likely reflect the different growth demands in the two conditions, our experiment also identified hundreds of candidate genes for the observed differences in morphology and pathobiology between BF and PL. We found an overrepresentation of upregulated genes in transport, secondary metabolism, and cell wall and surface functions. Furthermore, upregulated genes showed significant spatial structure across the A. fumigatus genome; they were more likely to occur in subtelomeric regions and colocalized in 27 genomic neighborhoods, many of which overlapped with known or candidate secondary metabolism gene clusters. We also identified 1,164 genes that were downregulated. This gene set was not spatially structured across the genome and was overrepresented in genes participating in primary metabolic functions, including carbon and amino acid metabolism. These results add valuable insight into the genetics of biofilm formation in A. fumigatus and other filamentous fungi and identify many relevant, in the context of biofilm biology, candidate genes for downstream functional experiments.
Authors:
John G Gibbons; Anne Beauvais; Remi Beau; Kriston L McGary; Jean-Paul Latgé; Antonis Rokas
Related Documents :
22212906 - Analysis of the apx, pgd1 and r1g1b constitutive gene promoters in various organs over ...
11859076 - Vav1 couples t cell receptor to serum response factor-dependent transcription via a mek...
12802426 - Functional association of immediate early gene c-fos with nuclear matrix.
17378576 - Identification of marker genes for intestinal immunomodulating effect of a fructooligos...
12052216 - Cytochrome p450 gene-directed enzyme prodrug therapy (gdept) for cancer.
2544026 - Control of gene expression by artificial introns in saccharomyces cerevisiae.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-07-01
Journal Detail:
Title:  Eukaryotic cell     Volume:  11     ISSN:  1535-9786     ISO Abbreviation:  Eukaryotic Cell     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-30     Completed Date:  2012-04-13     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  101130731     Medline TA:  Eukaryot Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  68-78     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aspergillus fumigatus / genetics*,  growth & development,  physiology*
Biofilms*
Cell Wall / genetics
Chromosome Mapping
Drug Resistance, Fungal / genetics
Fungal Proteins / genetics,  metabolism
Gene Expression
Gene Expression Regulation, Fungal
Gene Regulatory Networks
Glycolysis / genetics
Metabolic Networks and Pathways / genetics
Multigene Family
Protein Biosynthesis
Ribosomes / genetics
Transcription Factors / genetics,  metabolism
Transcriptome*
Grant Support
ID/Acronym/Agency:
F31AI091343-01/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Fungal Proteins; 0/Transcription Factors
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A genomewide screen for tolerance to cationic drugs reveals genes important for potassium homeostasi...
Next Document:  Calcineurin colocalizes with P-bodies and stress granules during thermal stress in Cryptococcus neof...