Document Detail

Global Identification of Small RNA Targets in Plants by Sequencing Sliced Ends of Messenger RNAs.
MedLine Citation:
PMID:  23135849     Owner:  NLM     Status:  In-Data-Review    
Small RNAs (microRNAs and other classes of endogenous small interfering RNAs) play important roles in a wide variety of biological processes. However, integration of small RNAs in diverse biological networks relies on the confirmation of their RNA targets. In plants, miRNAs negatively regulate mRNA targets by guiding a cleavage in the complementary site that leaves a 3' polyadenylated RNA possessing monophosphate at its 5' end. This chapter describes a detailed step-by-step protocol for cloning such sliced 3' products in order to identify small RNA targets. Using this protocol, we have identified more than 150 small RNA targets in rice; some are conserved and others are non-conserved targets for rice small RNAs.
Yong-Fang Li; Ramanjulu Sunkar
Related Documents :
24201489 - Dna variations in regenerated plants of pea (pisum sativum l.).
25092339 - Short sequences can efficiently recruit histone h3 lysine 27 trimethylation in the abse...
3971349 - Dna ethylation in target and non-target organs of hamsters and rats treated with diethy...
23021549 - Development of novel visual-plus quantitative analysis systems for studying dna double-...
24106089 - Comprehensive analysis of dna polymerase iii α subunits and their homologs in bacteria...
11999389 - Simple cryoprotection and cell dissociation techniques for application of the comet ass...
20541619 - Fused in sarcoma/translocated in liposarcoma: a multifunctional dna/rna binding protein.
15452279 - A general role of the dna glycosylase nth1 in the abasic sites cleavage step of base ex...
16352529 - Herpes simplex virus dna synthesis is not a decisive regulatory event in the initiation...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  956     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  119-29     Citation Subset:  IM    
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cloning of Small RNAs for the Discovery of Novel MicroRNAs in Plants.
Next Document:  Artificial MicroRNAs for Specific Gene Silencing in Rice.