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Global Gene Expression Profiling in Infants with Acute Respiratory Syncytial Virus Broncholitis Demonstrates Systemic Activation of Interferon Signaling Networks.
MedLine Citation:
PMID:  23190772     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND:: Respiratory syncytial virus (RSV) is a leading cause of pediatric lower respiratory tract infections and has a high impact on pediatric emergency department (ED) utilization. Variation in host response may influence the pathogenesis and disease severity. We evaluated global gene expression profiles to better understand the systemic host response to acute RSV bronchiolitis in infants and young children. METHODS:: Patients (age ≤ 24 months) who were clinically diagnosed with acute bronchiolitis and who had a positive rapid test for RSV assay were recruited from the Texas Children's Hospital ED. Global gene expression of peripheral whole blood cells were analyzed in 21 cases and 37 age-matched healthy controls. Transcripts exhibiting significant upregulation and downregulation as a result of RSV infection were identified and confirmed in a subset of samples using RNAseq. The potential pathways affected were analyzed. RESULTS:: Blood was obtained from patients with acute RSV bronchiolitis (mean age 6 months). Of these, 43% were admitted to the hospital, 52% were given intravenous fluids and 24% received oxygen. Highly significant expression differences were detected in a discovery cohort of White infants (N=33) and validated in an independent group of African American infants (N=19). Individuals with mild disease (N=15) could not be distinguished from subjects with clinically moderate disease (N=5). Pathway enrichment analyses of the differentially expressed genes demonstrated extensive activation of the innate immune response, particularly the interferon signaling network. There was a significant downregulation of transcripts corresponding to antigen presentation.
Authors:
Kristine L Bucasas; Asad I Mian; Gail J Demmler-Harrison; Alison C Caviness; Pedro A Piedra; Luis M Franco; Chad A Shaw; Yijie Zhai; Xueqing Wang; Molly S Bray; Robert B Couch; John W Belmont
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-28
Journal Detail:
Title:  The Pediatric infectious disease journal     Volume:  -     ISSN:  1532-0987     ISO Abbreviation:  Pediatr. Infect. Dis. J.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701858     Medline TA:  Pediatr Infect Dis J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1 Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, 2 Department of Pediatrics, Baylor College of Medicine, Houston, Texas, 3 Departments of Molecular Virology & Microbiology and Medicine, Baylor College of Medicine, Houston, Texas, 4 Department of Medicine, Baylor College of Medicine, Houston, Texas, 5 Department of Epidemiology, University of Alabama, Birmingham, Alabama.
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