| Global DNA methylation levels in white blood cell DNA from sisters discordant for breast cancer from the New York site of the Breast Cancer Family Registry. | |
| | |
MedLine Citation:
|
PMID: 22705975 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Lower global DNA methylation is associated with genomic instability and it is one of the epigenetic mechanisms relevant to carcinogenesis. Emerging evidence for several cancers suggests that lower overall levels of global DNA methylation in blood are associated with different cancer types, although less is known about breast cancer. We examined global DNA methylation levels using a sibling design in 273 sisters affected with breast cancer and 335 unaffected sisters from the New York site of the Breast Cancer Family Registry. We measured global DNA methylation in total white blood cell (WBC) and granulocyte DNA by two different methods, the [ ( 3) H]-methyl acceptance assay and the luminometric methylation assay (LUMA). Global methylation levels were only modestly correlated between sisters discordant for breast cancer (Spearman correlation coefficients ranged from -0.08 to 0.24 depending on assay and DNA source). Using conditional logistic regression models, women in the quartile with the lowest DNA methylation levels (as measured by the [ ( 3) H]-methyl acceptance assay) had a 1.8-fold (95% CI = 1.0-3.3) higher relative association with breast cancer than women in the quartile with the highest DNA methylation levels. When we examined the association on a continuous scale, we also observed a positive association (odds ratio, OR = 1.3, 95% CI = 1.0-1.7, for a one unit change in the natural logarithm of the DPM/μg of DNA). We observed no association between measures by the LUMA assay and breast cancer risk. If replicated in prospective studies, this study suggests that global DNA methylation levels measured in WBC may be a potential biomarker of breast cancer risk even within families at higher risk of cancer. |
| | |
Authors:
|
Lissette Delgado-Cruzata; Hui-Chen Wu; Mary Perrin; Yuyan Liao; Maya A Kappil; Jennifer S Ferris; Julie D Flom; Hulya Yazici; Regina M Santella; Mary Beth Terry |
Related Documents
:
|
21141345 - Mesothelioma and asbestos, fifty years of evidence: chris wagner and the contribution o... 22527495 - Epispot assay: detection of viable dtcs/ctcs in solid tumor patients. 22428115 - Are lycopene metabolites metabolically active? 22719195 - Xaf1 is frequently methylated in human esophageal cancer. 10197605 - Superiority of yeast over bacterial cytosine deaminase for enzyme/prodrug gene therapy ... 11763245 - A psychometric analysis of the quality of life-cancer survivors (qol-cs) in survivors o... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-06-18 |
Journal Detail:
|
Title: Epigenetics : official journal of the DNA Methylation Society Volume: 7 ISSN: 1559-2308 ISO Abbreviation: Epigenetics Publication Date: 2012 Aug |
Date Detail:
|
Created Date: 2012-08-28 Completed Date: 2013-01-14 Revised Date: 2013-04-16 |
Medline Journal Info:
|
Nlm Unique ID: 101265293 Medline TA: Epigenetics Country: United States |
Other Details:
|
Languages: eng Pagination: 868-74 Citation Subset: IM |
Affiliation:
|
Department of Epidemiology, Mailman School of Public Health of Columbia University, New York, NY, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Breast Neoplasms / epidemiology, genetics* DNA / blood* DNA Methylation* Female Humans Leukocytes / metabolism* Middle Aged New York / epidemiology Registries Siblings |
| Grant Support | |
ID/Acronym/Agency:
|
CA-06-503/CA/NCI NIH HHS; K07 CA131094/CA/NCI NIH HHS; K07 CA131094/CA/NCI NIH HHS; L30 HG004809/HG/NHGRI NIH HHS; P30 CA13696/CA/NCI NIH HHS; P30 ES009089/ES/NIEHS NIH HHS; U01 CA69398/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Protodecarboxylation of benzoic acids under radical conditions.
Next Document: Plastic flies: The regulation and evolution of trait variability in Drosophila.