Document Detail


Global DNA methylation: Uncommon event in Oral Lichenoid Disease.
MedLine Citation:
PMID:  24724918     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVES: Accumulating evidence indicates that aberrant DNA methylation is closely related to oral carcinogenesis, and it has been shown that methylation changes might be used as prognostic biomarker in oral squamous cell carcinoma. Oral lichenoid disease (OLD) is the most common oral potentially malignant disorder in our region. The aim of this study was to perform the first wide DNA methylation study in OLD in order to investigate the relevance of DNA methylation changes in this premalignant disorder.
MATERIALS AND METHODS: Two different Illumina microarray platforms, namely the GoldenGate Cancer Panel I and the HumanMethylation27 DNA Analysis BeadChip, were utilized in the discovery phase to interrogate the methylation profile of 59 OLD cases and 9 healthy individuals. Top ranked genes were further validated by pyrosequencing in a second sample set consisting of 160 OLD and 65 controls.
RESULTS: Our results show that the frequency of aberrant DNA methylation is rare in OLD, and this finding was further corroborated by pyrosequencing in the biological validation.
CONCLUSIONS: These findings reinforce the notion that molecular alterations associated with oral carcinogenesis do not seem to be common events in OLD, which in turn might explain the low rate of malignization of this disorder. This article is protected by copyright. All rights reserved.
Authors:
Naiara G Bediaga; Xabier Marichalar-Mendia; Jose M Aguirre-Urizar; Borja Calvo; María A Echebarria-Goicouria; Marian M de Pancorbo; Amelia Acha-Sagredo
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-4-12
Journal Detail:
Title:  Oral diseases     Volume:  -     ISSN:  1601-0825     ISO Abbreviation:  Oral Dis     Publication Date:  2014 Apr 
Date Detail:
Created Date:  2014-4-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508565     Medline TA:  Oral Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
This article is protected by copyright. All rights reserved.
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