Document Detail


Glioma morphology and tumor-induced vascular alterations revealed in seven rodent glioma models by in vivo magnetic resonance imaging and angiography.
MedLine Citation:
PMID:  20677250     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To evaluate the added value of non-contrast-enhanced MR angiography (MRA) to conventional MR imaging for a detailed characterization of different rodent glioma models.
MATERIALS AND METHODS: Intracerebral tumor cell implantation and chemical induction methods were implemented to obtain rat C6, 9L/LacZ, F98, RG2, and ethyl-nitrosourea (ENU) -induced glioma models, a human U87 MG tumor model as well as a mouse GL261 glioma model. MR assessments were regularly conducted on a 7 Tesla Bruker BioSpin system. The tumor border sharpness and growth characteristics of each glioma model were assessed from T(2)-weighted images. Neovascularization and vascular alterations inherent to each model were characterized by assessing absolute blood volumes, vessel density, length, and diameter using Mathematica and Amira software.
RESULTS: The 9L/LacZ and ENU gliomas both presented flaws that hinder their use as reliable brain tumor models. C6 gliomas were slightly invasive and induced moderate vascular alterations, whereas GL261 tumors dramatically altered the brain vessels in the glioma region. F98, RG2, and U87 are infiltrative models that produced dramatic vascular alterations.
CONCLUSION: MRI and MRA provided crucial in vivo information to identify a distinctive "fingerprint" for each of our seven rodent glioma models.
Authors:
Sabrina Doblas; Ting He; Debbie Saunders; Jamie Pearson; Jessica Hoyle; Nataliya Smith; Megan Lerner; Rheal A Towner
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of magnetic resonance imaging : JMRI     Volume:  32     ISSN:  1522-2586     ISO Abbreviation:  J Magn Reson Imaging     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-02     Completed Date:  2010-11-24     Revised Date:  2011-08-03    
Medline Journal Info:
Nlm Unique ID:  9105850     Medline TA:  J Magn Reson Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  267-75     Citation Subset:  IM    
Copyright Information:
2010 Wiley-Liss, Inc.
Affiliation:
Advanced Magnetic Resonance Center, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Neoplasms / chemically induced,  pathology*
Cell Line, Tumor
Disease Models, Animal
Female
Glioma / chemically induced,  pathology*
Humans
Magnetic Resonance Angiography / methods*
Magnetic Resonance Imaging / methods*
Male
Mice
Neoplasm Transplantation
Neovascularization, Pathologic
Rats
Rats, Inbred F344
Grant Support
ID/Acronym/Agency:
P20 RR016478-055473/RR/NCRR NIH HHS; P20 RR016478-067040/RR/NCRR NIH HHS; P20 RR016478-077278/RR/NCRR NIH HHS; P20RR016478/RR/NCRR NIH HHS
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