Document Detail

Glial swelling during extracellular acidosis in vitro.
MedLine Citation:
PMID:  3354026     Owner:  NLM     Status:  MEDLINE    
Intracellular and extracellular acidosis may determine the ultimate outcome for brain tissue in cerebral ischemia. An extracellular acidosis that occurs in the penumbra zone was investigated in vitro as to its role in the formation of cytotoxic cell swelling. For that purpose, C6 glioma cells or primary cultured astrocytes were suspended in normal isotonic medium in normoxia during acidification to a final pH of 6.2. The cell volume response was determined by flow cytometry using hydrodynamic focusing, which allows one to recognize changes in cell size of less than 1%. A threshold pH of 6.8 was found that had to be crossed to induce cell swelling by acidosis. Once pH fell below this threshold, the increase in cell size appeared to be an all-or-nothing phenomenon. The cells rapidly assumed a final cell size of 115% of normal in the case of C6 glioma or of 118% in the case of primary cultured astrocytes independent of the actual level of acidosis or the duration of exposure. Acidosis-induced glial swelling could be significantly attenuated by 1) addition of amiloride, 2) administration of acetazolamide, or 3) replacement of bicarbonate buffer against N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES). Replacement of extracellular Na+ by choline chloride led to complete prevention of the acidosis-induced cell swelling. Taken together, the findings strongly indicate a central involvement of Na+/H+ and Cl-/HCO3- exchange mechanisms in the development of cell swelling under these conditions. Activation of the Na+/H+ antiporter can be considered an attempt to maintain a normal intracellular pH at the expense of an abnormal cell volume.(ABSTRACT TRUNCATED AT 250 WORDS)
O Kempski; F Staub; M Jansen; F Schödel; A Baethmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stroke; a journal of cerebral circulation     Volume:  19     ISSN:  0039-2499     ISO Abbreviation:  Stroke     Publication Date:  1988 Mar 
Date Detail:
Created Date:  1988-05-02     Completed Date:  1988-05-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0235266     Medline TA:  Stroke     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  385-92     Citation Subset:  IM    
Institute for Surgical Research, Klinikum Grosshadern, Ludwig-Maximilians-University, München, Federal Republic of Germany.
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MeSH Terms
Acidosis / pathology*
Alkalosis / pathology
Amiloride / pharmacology
Extracellular Space
HEPES / pharmacology
Hydrogen-Ion Concentration
Neuroglia / pathology*
Tumor Cells, Cultured
Reg. No./Substance:
2609-46-3/Amiloride; 7365-45-9/HEPES

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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