Document Detail

Glial-derived neurotrophic factor in human adult and fetal intestine and in Hirschsprung's disease.
MedLine Citation:
PMID:  9098026     Owner:  NLM     Status:  MEDLINE    
BACKGROUND & AIMS: Glial cell line-derived neurotrophic factor (GDNF) signals through the product of the ret proto-oncogene, which is known to be mutated in Hirschsprung's disease and other conditions with gut dysmotility. The aim of this study was to determine the presence of GDNF and its receptor component (RET) in human adult and developing intestine and to evaluate their distribution pattern in Hirschsprung's disease.
METHODS: GDNF and RET were detected immunohistochemically, and GDNF was quantified by immunoassay in specimens of human adult and fetal colon and Hirschsprung's disease intestine.
RESULTS: GDNF-like immunoreactivity was detected in all specimens. Immunostaining of GDNF was restricted to neural fiber-like structures across the gut wall and was similar to staining with markers of glia and Schwann cells. In contrast, RET immunoreactivity was found only in neural cell bodies. GDNF levels determined by immunoassay were higher in muscle than mucosal gut layers, and there was no difference between affected and unaffected segments of Hirschsprung's disease.
CONCLUSIONS: GDNF is present in adult and fetal human gut, where it may play a neurotrophic role. Its staining pattern suggests that it is localized in glia or Schwann cells. There seems to be no difference of GDNF levels between affected and unaffected intestinal segments in Hirschsprung's disease.
K J Bär; P Facer; N S Williams; P K Tam; P Anand
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gastroenterology     Volume:  112     ISSN:  0016-5085     ISO Abbreviation:  Gastroenterology     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-05-01     Completed Date:  1997-05-01     Revised Date:  2012-06-04    
Medline Journal Info:
Nlm Unique ID:  0374630     Medline TA:  Gastroenterology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1381-5     Citation Subset:  AIM; IM    
Department of Neurology, Saint Bartholomew's and The Royal London Hospital School of Medicine and Dentistry, England.
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MeSH Terms
Drosophila Proteins*
Fetus / metabolism*
Glial Cell Line-Derived Neurotrophic Factor
Glial Cell Line-Derived Neurotrophic Factor Receptors
Glial Fibrillary Acidic Protein / metabolism
Hirschsprung Disease / metabolism*
Intestines / innervation,  metabolism*
Nerve Fibers / metabolism
Nerve Growth Factors / metabolism
Nerve Tissue Proteins / metabolism*
Proto-Oncogene Proteins / metabolism
Proto-Oncogene Proteins c-ret
Receptor Protein-Tyrosine Kinases / metabolism
Reference Values
Reg. No./Substance:
0/Drosophila Proteins; 0/GDNF protein, human; 0/Glial Cell Line-Derived Neurotrophic Factor; 0/Glial Cell Line-Derived Neurotrophic Factor Receptors; 0/Glial Fibrillary Acidic Protein; 0/Nerve Growth Factors; 0/Nerve Tissue Proteins; 0/Proto-Oncogene Proteins; EC Proteins c-ret; EC Protein-Tyrosine Kinases; EC oncogene protein, Drosophila

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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