Document Detail

Glial cell line-derived neurotrophic factor in Purkinje cells of adult zebrafish: an autocrine mode of action?
MedLine Citation:
PMID:  19732805     Owner:  NLM     Status:  MEDLINE    
Glial cell line-derived neurotrophic factor (GDNF) has a neuroprotective role in Purkinje cells of cerebellum, promoting the survival and the differentiation of these cells. Its signalling is mediated by a receptorial complex GFRalpha1/RET. In the brain of adult zebrafish (Danio rerio) we previously investigated GDNF expression and localization, but no data exist regarding GFRalpha1 and RET presence. Thus, the present study was designed to clarify the morphological relation between GDNF and its receptorial complex GFRalpha1/RET immunoreactivity in the cerebellum of adult zebrafish. The expression of gdnf, GFRalpha1 and ret genes was demonstrated in adult zebrafish cerebellum by a standard RT-PCR. The distribution of GDNF and its receptorial complex GFRalpha1/RET was examined by single and double immunocytochemical stainings. In the valvula and corpus cerebelli GDNF, GFRalpha1 and RET immunoreactivity was seen co-localized in Purkinje cells, identified morphologically and by using an antiserum against a specific marker for these cells, aldolase C enzyme. In the vestibulolateralis lobe, Purkinje neurons were lacking in both the eminentiae granulares and medial caudal lobe. These results demonstrated the expression of the GDNF receptorial complex in adult zebrafish cerebellum and suggest an autocrine mode of action of GDNF in Purkinje cells.
Bruna Facello; Luciana Castaldo; Luisa De Martino; Carla Lucini
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Publication Detail:
Type:  Journal Article     Date:  2009-09-02
Journal Detail:
Title:  Neuroscience letters     Volume:  465     ISSN:  1872-7972     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-09-30     Completed Date:  2010-01-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  133-7     Citation Subset:  IM    
Dpt. Biological Structures, Functions and Technology, University of Naples Federico II, 80137 Naples, Italy.
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MeSH Terms
Cerebellum / metabolism*
Fructose-Bisphosphate Aldolase / metabolism
Glial Cell Line-Derived Neurotrophic Factor / metabolism*
Glial Cell Line-Derived Neurotrophic Factor Receptors / metabolism
Proto-Oncogene Proteins c-ret / metabolism
Purkinje Cells / metabolism*
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Zebrafish Proteins / metabolism
Reg. No./Substance:
0/GFRalpha1 protein, zebrafish; 0/Glial Cell Line-Derived Neurotrophic Factor; 0/Glial Cell Line-Derived Neurotrophic Factor Receptors; 0/RNA, Messenger; 0/Zebrafish Proteins; EC Proteins c-ret; EC protein, zebrafish; EC Aldolase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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