| Glial cell line-derived neurotrophic factor stimulates the morphological differentiation of cultured ventral mesencephalic calbindin- and calretinin-expressing neurons. | |
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MedLine Citation:
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PMID: 10877917 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glial cell line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor for mesencephalic dopaminergic neurons. Subpopulations of these neurons express the calcium-binding proteins calbindin (CB) and calretinin (CR). Understanding the specific effects of GDNF on these neurons is important for the development of an optimal cell replacement therapy for Parkinson's disease. To investigate the effects of GDNF on the morphological complexity of mesencephalic tyrosine hydroxylase (TH)-immunoreactive (-ir), CB-ir, and CR-ir neurons, dissociated cultures of embryonic (E14) rat ventral mesencephalon were prepared. Chronic administration of GDNF (10 ng/ml) for 7 days promoted the survival of TH-ir and CB-ir neurons but did not alter the density of CR-ir neurons. Total fiber length/neuron and number of branching points/neuron of CB-ir and CR-ir cells were significantly increased after GDNF treatment (2x for CB-ir cells and 1.4x and 1.7x, respectively, for CR-ir cells), which resulted in a significantly larger size of neurite field/neuron (2.9x and 1.5x for CB-ir and CR-ir neurons, respectively). The number of primary neurites/neuron of CB-ir neurons was found to be 1.5x larger, while no difference could be detected for CR-ir cells. Assessment of the effects of GDNF on TH-ir neurons unveiled a similar outcome with an increased total fiber length/neuron (1.5x), an increased number of primary neurites/neuron (1.6x), and a twofold larger size of neurite field/neuron. In conclusion, our findings recognize GDNF as a neurotrophic factor that stimulates the morphological differentiation of ventral mesencephalic CB-ir and CR-ir neurons. |
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Authors:
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H R Widmer; B Schaller; M Meyer; R W Seiler |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental neurology Volume: 164 ISSN: 0014-4886 ISO Abbreviation: Exp. Neurol. Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2000-08-16 Completed Date: 2000-08-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370712 Medline TA: Exp Neurol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 71-81 Citation Subset: IM |
Copyright Information:
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Copyright 2000 Academic Press. |
Affiliation:
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Department of Neurosurgery, University of Bern, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium-Binding Protein, Vitamin D-Dependent / biosynthesis* Cell Differentiation / drug effects* Cell Survival / drug effects Cells, Cultured Glial Cell Line-Derived Neurotrophic Factor Mesencephalon / drug effects*, embryology, metabolism Nerve Growth Factors* Nerve Tissue Proteins / pharmacology* Neurites / drug effects Neurons / cytology, drug effects*, metabolism Neuroprotective Agents / pharmacology Rats Rats, Sprague-Dawley Tyrosine 3-Monooxygenase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Gdnf protein, rat; 0/Glial Cell Line-Derived Neurotrophic Factor; 0/Nerve Growth Factors; 0/Nerve Tissue Proteins; 0/Neuroprotective Agents; 0/calbindin; 0/calretinin; EC 1.14.16.2/Tyrosine 3-Monooxygenase |
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