Document Detail

Glia-derived nexin potentiates neurite extension in hippocampal pyramidal cells in vitro.
MedLine Citation:
PMID:  2335138     Owner:  NLM     Status:  MEDLINE    
Glia-derived nexin (GDN) is a serine protease inhibitor which promotes the outgrowth of neurites from neuroblastoma cells and from chick sympathetic neurons. However, it has not been demonstrated that this effect is independent of its protease inhibitory activity. We report here that, 48 h after the addition of GDN to astrocyte-free cultures of rat hippocampal cells, there was a significant increase in axon length, although dendrite length and the total number of neurites were unaffected. Nerve growth factor added alone had no significant effect nor was there any additional effect when it was added together with GDN. However, hirudin, a thrombin inhibitor purified from leeches, was found to mimic the GDN effect at similar molar concentrations of active protein. This suggests that the protease inhibitory activity is crucial for the neurite-promoting effect of GDN on hippocampal neurons.
L Farmer; J Sommer; D Monard
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Developmental neuroscience     Volume:  12     ISSN:  0378-5866     ISO Abbreviation:  Dev. Neurosci.     Publication Date:  1990  
Date Detail:
Created Date:  1990-06-14     Completed Date:  1990-06-14     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7809375     Medline TA:  Dev Neurosci     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  73-80     Citation Subset:  IM    
Friedrich Miescher Institute, Basel, Switzerland.
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MeSH Terms
Amyloid beta-Protein Precursor
Carrier Proteins / pharmacology*
Cells, Cultured
Dendrites / drug effects*
Hippocampus / cytology*,  drug effects,  physiology
Nerve Growth Factors / metabolism,  pharmacology*
Neuroglia / metabolism*
Receptors, Cell Surface
Serine Proteinase Inhibitors / metabolism,  pharmacology*
Reg. No./Substance:
0/Amyloid beta-Protein Precursor; 0/Carrier Proteins; 0/Nerve Growth Factors; 0/Receptors, Cell Surface; 0/Serine Proteinase Inhibitors; 0/protease nexins

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