Document Detail


Glass beads load macromolecules into living cells.
MedLine Citation:
PMID:  2459146     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe and characterize an exceptionally rapid and simple new technique for loading large numbers of cultured cells with large macromolecules. The culture medium of the cell monolayer is replaced by a small volume of the macromolecule to be loaded. Glass beads (75-500 micron diameter) are then sprinkled onto the cells, the cells are washed free of beads and exogenous macromolecules, and 'bead-loading' is completed. The conditions for bead-loading can readily be modified to accommodate cell type and loading objectives: for example, the amount of loading per cell increases if bead size is increased or if beads are agitated after sprinkling onto the monolayer, but at the expense of increased cell loss. As many as 97% of a population of bovine aortic endothelial (BAE) cells were loaded with a 10,000 Mr dextran; and 79% with a 150,000 Mr dextran using bead-loading. Various cell lines have been loaded using glass beads. Moreover, bead-loading has the advantage of producing loaded cells that remain adherent and well-spread, thus minimizing recovery time and permitting immediate microscopic examination.
Authors:
P L McNeil; E Warder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cell science     Volume:  88 ( Pt 5)     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-10-28     Completed Date:  1988-10-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  669-78     Citation Subset:  IM    
Affiliation:
Department of Anatomy & Cellular Biology, Harvard Medical School, Boston, MA 02115.
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MeSH Terms
Descriptor/Qualifier:
Cell Survival
Cells, Cultured*
Dextrans
Flow Cytometry
Glass*
Macromolecular Substances*
Grant Support
ID/Acronym/Agency:
R01 CA42275/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Macromolecular Substances; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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