| Ginsenoside Rg1 delays tert-butyl hydroperoxide-induced premature senescence in human WI-38 diploid fibroblast cells. | |
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MedLine Citation:
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PMID: 18375874 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tert-butyl hydroperoxide (t-BHP), an analog of hydroperoxide, induced characteristic changes of senescence in human diploid fibroblasts WI-38 cells. It was reported that ginsenoside Rg1, an active ingredient of ginseng, ameliorated learning deficits in aged rats. The present study was aimed to investigate whether ginsenoside Rg1 can delay the premature senescence of WI-38 cells induced by t-BHP and to explore the underlying molecular mechanisms. First, Rg1 pretreatment markedly reversed senescent morphological changes in WI-38 cells induced by t-BHP. Second, t-BHP treatment alone resulted in an increase in the protein levels of P16 and P21, and a decline in intracellular adenosine 5'-triphosphate (ATP) level and mitochondrial complex IV activity. Ginsenoside Rg1 pretreatment had significant effects of attenuating these changes. These data indicate that ginsenoside Rg1 has an anti-aging effect on t-BHP-induced premature senescence in WI-38 cells. This effect may be mediated by regulating cell cycle proteins and enhancing mitochondrial functioning. |
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Authors:
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Xiaochun Chen; Jing Zhang; Yaxiu Fang; Chaohui Zhao; Yuangui Zhu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The journals of gerontology. Series A, Biological sciences and medical sciences Volume: 63 ISSN: 1079-5006 ISO Abbreviation: J. Gerontol. A Biol. Sci. Med. Sci. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-31 Completed Date: 2008-06-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9502837 Medline TA: J Gerontol A Biol Sci Med Sci Country: United States |
Other Details:
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Languages: eng Pagination: 253-64 Citation Subset: AIM; IM |
Affiliation:
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Fujian Institute of Geriatrics, The Affiliated Union Hospital of Fujian Medical University, Fuzhou, Fujian 350001, PR China. chenxiaochun998@gmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Blotting, Western Cell Aging / drug effects* Cell Line Cell Line, Tumor Cell Survival / drug effects Cyclin-Dependent Kinase Inhibitor p16 / metabolism Cyclin-Dependent Kinase Inhibitor p21 / metabolism Diploidy Dose-Response Relationship, Drug Electron Transport / drug effects Enzyme-Linked Immunosorbent Assay Fibroblasts / cytology, drug effects*, metabolism Gene Expression / drug effects Ginsenosides / chemistry, pharmacology* Humans Lysosomes / drug effects, ultrastructure Microscopy, Electron, Transmission Mitochondria / drug effects, metabolism, ultrastructure Molecular Structure Reverse Transcriptase Polymerase Chain Reaction Telomerase / genetics, metabolism tert-Butylhydroperoxide / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Cyclin-Dependent Kinase Inhibitor p16; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Ginsenosides; 22427-39-0/ginsenoside Rg1; 56-65-5/Adenosine Triphosphate; 75-91-2/tert-Butylhydroperoxide; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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