Document Detail


Ginsenoside RH-2 induces apoptotic cell death in rat C6 glioma via a reactive oxygen- and caspase-dependent but Bcl-X(L)-independent pathway.
MedLine Citation:
PMID:  10447219     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We used the rat C6 gliomal cell line to investigate the potential role of ginsenoside Rh2 (G-Rh2) in brain tumor. G-Rh2 induced many apoptotic manifestations in C6 gliomal cells as evidenced by changes in cell morphology, generation of DNA fragmentation, activation of caspase and production of reactive oxygen species (ROS). As a result, cotreatment with antioxidants or a broad-spectrum caspase inhibitor, N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone effectively attenuated G-Rh2-induced cell death. However, specific cleavage of poly(ADP-ribose)polymerase into 85 kDa protein was not detected as demonstrated in many other apoptotic paradigms. Expression levels of Bcl-2 and Bax remained unchanged following G-Rh2 treatment. Furthermore, G-Rh2-induced cell death in C6 gliomal cells overexpressing antiapoptotic protein, Bcl-X(L), was comparable to that in parental cells. Taken together, our data indicate that G-Rh2-induced cell death is mediated by the generated ROS and the activation of caspase pathway in a Bcl-X(L)-independent manner.
Authors:
H E Kim; J H Oh; S K Lee; Y J Oh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Life sciences     Volume:  65     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  1999  
Date Detail:
Created Date:  1999-08-27     Completed Date:  1999-08-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  PL33-40     Citation Subset:  IM    
Affiliation:
Department of Biology, Yonsei University College of Science, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Caspases / metabolism*
DNA Fragmentation / drug effects
Enzyme Activation
Ginsenosides*
Glioma
Immunoblotting
Oxidative Stress / drug effects
Panax / chemistry*
Plants, Medicinal*
Proto-Oncogene Proteins / biosynthesis
Proto-Oncogene Proteins c-bcl-2 / metabolism*
Rats
Reactive Oxygen Species / metabolism*
Saponins / pharmacology*
Tetrazolium Salts
Thiazoles
Transfection
Tumor Cells, Cultured
bcl-2-Associated X Protein
bcl-X Protein
Chemical
Reg. No./Substance:
0/Bax protein, rat; 0/Bcl2l1 protein, rat; 0/Ginsenosides; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Reactive Oxygen Species; 0/Saponins; 0/Tetrazolium Salts; 0/Thiazoles; 0/bcl-2-Associated X Protein; 0/bcl-X Protein; 298-93-1/thiazolyl blue; 78214-33-2/ginsenoside Rh2; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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