Document Detail


Ginkgo biloba extract prevents against apoptosis induced by high glucose in human lens epithelial cells.
MedLine Citation:
PMID:  18718173     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate the protective effects of Ginkgo biloba extract (GBE) on high glucose-induced apoptosis of human lens epithelial cells (HLEC) and the possible molecular mechanisms. METHODS: The cultured HLEC were allotted into 6 groups: normal group, high glucose group, low-, moderate-, and high-dose GBE group, and the bendazac lysine group. Cell viability, cell apoptosis, the activities of cell antioxidases, aldose reductase, caspase-3, the levels of cell antioxidants, and the expressions of Bcl-2 and Bax were assessed by different methods. RESULTS: After being incubated with high glucose for 24 h, HLEC underwent apoptosis and exhibited significant oxidative stress. In the presence of GBE at different doses, the rate of HLEC apoptosis was lower and the oxidative stress state was significantly ameliorated. The increased ratio of Bax to Bcl-2 was significantly reduced and the activation of caspase-3 was suppressed by GBE in a dose-dependent manner. CONCLUSION: GBE prevents HLEC from high glucose-induced apoptosis through inhibiting oxidative stress, reducing the ratio of Bax to Bcl-2, and decreasing the activity of caspase-3. Therefore, GBE has a potential protective effect against diabetic cataract formation.
Authors:
Zheng-mei Wu; Xiao-xing Yin; Lei Ji; Yuan-yuan Gao; Ying-mei Pan; Qian Lu; Jian-yun Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  29     ISSN:  1745-7254     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-22     Completed Date:  2009-05-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  1042-50     Citation Subset:  IM    
Affiliation:
Department of Clinical Pharmacology, Xuzhou Medical College, Xuzhou 221002, China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Apoptosis Regulatory Proteins / biosynthesis,  genetics
Cell Line
Cell Nucleus / drug effects,  ultrastructure
Cell Survival / drug effects
Dose-Response Relationship, Drug
Epithelial Cells / drug effects*
Ginkgo biloba / chemistry*
Glucose / antagonists & inhibitors*,  toxicity*
Humans
Lens, Crystalline / cytology*
Oxidative Stress / drug effects
Plant Extracts / pharmacology*
Chemical
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/Plant Extracts; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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