Document Detail


Gingival crevicular fluid biochemical markers in periodontal disease: a cross-sectional study.
MedLine Citation:
PMID:  20927426     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To evaluate the levels of interleukin-1b (IL-1b) and alkaline phosphatase (ALP) in gingival crevicular fluid (GCF) and correlate these measurements with clinical characteristics of healthy patients and patients with gingivitis and periodontitis.
METHOD AND MATERIALS: Thirty-six individuals were subdivided into three groups of 12: control, gingivitis, and periodontitis groups. GCF samples were obtained from 2 sites for each patient. GCF volume was measured with the Periotron 8000; IL-1b levels were evaluated using the ELISA technique; ALP was measured by the kinetic method.
RESULTS: Mean levels of IL-1b and ALP in the 3 groups were, respectively, 22.34 ± 16.53 ΜL/site and 7.68 ± 2.46 U/L in the control group; 41.46 ± 27.98 ΜL/site and 9.80 ± 1.53 U/L in the gingivitis group; and 105.97 ± 89.26 ΜL/site and 11.56 ± 1.82 U/L in the periodontitis group. The amount of IL-1b in the periodontitis group was significantly higher than in the other groups, but no significant difference was found between the control group and the gingivitis group. The amount of ALP differed significantly among the 3 groups (P < .05). A positive correlation was observed among IL-1b levels, GCF volume, and probing depth. There was no evidence for a correlation between IL-1b and ALP levels.
CONCLUSION: Monitoring immune markers may give additional information on healthy or diseased sites.
Authors:
Caroline Perozini; Priscilla C A Chibebe; Mariella Vieira Pereira Leao; Celso da Silva Queiroz; Debora Pallos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Quintessence international (Berlin, Germany : 1985)     Volume:  41     ISSN:  1936-7163     ISO Abbreviation:  Quintessence Int     Publication Date:    2010 Nov-Dec
Date Detail:
Created Date:  2010-10-07     Completed Date:  2011-02-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0342677     Medline TA:  Quintessence Int     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  877-83     Citation Subset:  D    
Affiliation:
Department of Periodontics, Universidade de Taubate, Taubate, Brazil. carolperozini@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Alkaline Phosphatase / analysis,  metabolism*
Analysis of Variance
Biological Markers / analysis
Case-Control Studies
Chronic Periodontitis / immunology,  metabolism*
Cross-Sectional Studies
Female
Gingival Crevicular Fluid / chemistry*
Gingivitis / immunology,  metabolism*
Humans
Interleukin-1beta / analysis,  metabolism*
Male
Middle Aged
Statistics, Nonparametric
Young Adult
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Interleukin-1beta; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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