| Gingival RAGE expression in calorie-restricted versus ad libitum-fed rats. | |
| | |
MedLine Citation:
|
PMID: 20572766 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
BACKGROUND: The receptor for advanced glycation end products (RAGE) is a multiligand receptor expressed in a number of cell types, including gingival epithelia. RAGE mediates inflammation and induces cellular oxidative stress. Upregulation of RAGE is associated with various diseases, such as periodontal and cardiovascular diseases. This study examines the hypothesis that the gingiva of rats fed a calorie-restriction (CR) diet expresses lower levels of RAGE than the gingiva of rats fed an ad libitum (AL) diet. METHODS: Male F344BN rats (n = 16) from the National Institute on Aging (NIA) were fed a CR (n = 8) or AL (n = 8) diet according to NIA recommendations. Rats were sacrificed by guillotine at 8 (n = 5), 18 (n = 3), 29 (n = 4), and 38 (n = 4) months of age. The gingiva from around the molars was dissected and submitted for histologic and molecular analyses. RESULTS: Immunohistochemistry revealed that RAGE was expressed in the plasma membrane and cytoplasm of gingival epithelial cells and endothelial cells from both groups. RAGE mRNA levels were quantified relative to levels of GAPDH mRNA by real-time reverse-transcriptase polymerase chain reaction. The mean relative RAGE mRNA level in the CR group (7.26 ± 0.54) was lower than in the AL group (10 ± 1.4) (P <0.05). There were no detectable differences in RAGE expression according to animal age. CONCLUSION: Gingival RAGE expression in rats is reduced by calorie restriction. |
| | |
Authors:
|
John P Aris; Megan C Elios; Enrique Bimstein; Shanon M Wallet; Seunghee Cha; Kesavalu N Lakshmyya; Joseph Katz |
Related Documents
:
|
20846516 - Kallikrein-related peptidase 6 in alzheimer's disease and vascular dementia. 8326826 - Amyloid precursor protein mrna levels in the mononuclear blood cells of alzheimer's and... 20205906 - A nuclear factor-binding domain in the 5'-untranslated region of the amyloid precursor ... 7519616 - Stable expression of heterologous microtubule-associated proteins (maps) in chinese ham... 19614926 - Oxygen deprivation inhibits basal keratinocyte proliferation in a model of human skin a... 12955496 - Role of tumor metastasis suppressor gene kai1 in digestive tract carcinomas and cancer ... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Journal of periodontology Volume: 81 ISSN: 1943-3670 ISO Abbreviation: J. Periodontol. Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-10-04 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8000345 Medline TA: J Periodontol Country: United States |
Other Details:
|
Languages: eng Pagination: 1481-7 Citation Subset: D; IM |
Affiliation:
|
Department of Anatomy and Cell Biology, College of Medicine, University of Florida, Gainesville, FL 32610-0414, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Grant Support | |
ID/Acronym/Agency:
|
AG023719/AG/NIA NIH HHS; U24 DE 016509/DE/NIDCR NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of irrigation with an antiseptic and oral administration of azithromycin on bacteremia cause...
Next Document: Adjunctive treatment of chronic periodontitis with daily dietary supplementation with omega-3 Fatty ...