Document Detail


Gigahertz frequency-domain fluorometry: resolution of complex decays, picosecond processes and future developments.
MedLine Citation:
PMID:  3148695     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe the principles, instrumentation and applications of frequency-domain fluorescence spectroscopy. This method is useful for the resolution of multi-exponential decays and complex anisotropy decays on the picosecond timescale. The present instrumentation allows measurements to 2 GHz, which has been used to measure rotational correlation times as short as 7 ps. In the future it may be possible to extend the frequency range to 10 GHz, which should allow still faster processes to be quantified. It should be emphasized that resolution of fast processes is not obtained at the expense of losing information on the nanosecond timescale. Additionally, the GHz frequency-domain measurements are performed using low excitation intensities, which do not damage the samples.
Authors:
J R Lakowicz; G Laczko; I Gryczynski; H Szmacinski; W Wiczk
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Journal of photochemistry and photobiology. B, Biology     Volume:  2     ISSN:  1011-1344     ISO Abbreviation:  J. Photochem. Photobiol. B, Biol.     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1989-05-10     Completed Date:  1989-05-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8804966     Medline TA:  J Photochem Photobiol B     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  295-311     Citation Subset:  IM    
Affiliation:
Department of Biological Chemistry, University of Maryland, School of Medicine, Baltimore 21201.
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MeSH Terms
Descriptor/Qualifier:
Lasers
Luminescent Measurements
Spectrometry, Fluorescence*
Grant Support
ID/Acronym/Agency:
GM 35154/GM/NIGMS NIH HHS; GM 39617/GM/NIGMS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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