| Giardia intestinalis escapes oxidative stress by colonizing the small intestine: A molecular hypothesis. | |
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MedLine Citation:
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PMID: 21280173 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Giardia intestinalis is the microaerophilic protozoon causing giardiasis, a common infectious intestinal disease. Giardia possesses an O(2) -scavenging activity likely essential for survival in the host. We report that Giardia trophozoites express the O(2) -detoxifying flavodiiron protein (FDP), detected by immunoblotting, and are able to reduce O(2) to H(2) O rapidly (∼3 μM O(2) × min × 10(6) cells at 37 °C) and with high affinity (C(50) = 3.4 ± 0.7 μM O(2) ). Following a short-term (minutes) exposure to H(2) O(2) ≥ 100 μM, the O(2) consumption by the parasites is irreversibly impaired, and the FDP undergoes a degradation, prevented by the proteasome-inhibitor MG132. Instead, H(2) O(2) does not cause degradation or inactivation of the isolated FDP. On the basis of the elevated susceptibility of Giardia to oxidative stress, we hypothesize that the parasite preferentially colonizes the small intestine since, compared with colon, it is characterized by a greater capacity for redox buffering and a lower propensity to oxidative stress. © 2011 IUBMB IUBMB Life, 63(1): 21-25, 2011. |
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Authors:
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Daniela Mastronicola; Alessandro Giuffrè; Fabrizio Testa; Antonella Mura; Elena Forte; Eugenio Bordi; Leopoldo Paolo Pucillo; Pier Luigi Fiori; Paolo Sarti |
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Publication Detail:
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Type: Journal Article Date: 2011-01-13 |
Journal Detail:
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Title: IUBMB life Volume: 63 ISSN: 1521-6551 ISO Abbreviation: IUBMB Life Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888706 Medline TA: IUBMB Life Country: England |
Other Details:
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Languages: eng Pagination: 21-5 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Wiley Periodicals, Inc. |
Affiliation:
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Department of Biochemical Sciences, CNR Institute of Molecular Biology and Pathology and Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza University of Rome, Rome, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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