| Giant yeast cells with nonrecyclable ribonucleotide reductase. | |
| | |
MedLine Citation:
|
PMID: 21442328 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Ribonucleotide reductase (RNR) catalyzes the reduction of ribonucleotides to deoxyribonucleotides and thereby provides the precursors required for DNA synthesis and repair. In an attempt to test cell resistance to a permanent replicational stress, we constructed a mutant Saccharomyces cerevisiae strain containing exclusively nonrecyclable catalytic subunits of RNR that become inactivated following the reduction of one ribonucleoside diphosphate. In this rnr1C883A rnr3Δ mutant, the synthesis of each deoxyribonucleotide thus requires the production of one Rnr1C883A protein, which means that 26 million Rnr1C883A proteins (half the protein complement of a wild-type cell) have to be produced during each cell cycle. rnr1C883A rnr3Δ cells grow under constant replicational stress, as evidenced by the constitutive activation of the checkpoint effector Rad53, and their S phase is considerably extended compared to the wild type. rnr1C883A rnr3Δ mutants also display additional abnormalities such as a median cell volume increased by a factor of 8, and the presence of massive inclusion bodies. However, they exhibit a good plating efficiency and can be propagated indefinitely. rnr1C883A rnr3Δ cells, which can be used as a protein overexpression system, thus illustrate the robustness of S. cerevisiae to multiple physiological parameters. |
| | |
Authors:
|
Emilie Ma; Arach Goldar; Jean-Marc Verbavatz; Marie-Claude Marsolier-Kergoat |
Related Documents
:
|
6780168 - Localization of the dicarboxylate binding protein in the cell envelope of escherichia c... 8181778 - Effect of ethanol on escherichia coli cells. enhancement of dna synthesis due to ethano... 9294448 - A mutation in the ftsk gene of escherichia coli affects cell-cell separation, stationar... 8408018 - Regulation of folate and one-carbon metabolism in mammalian cells. i. folate metabolism... 23314638 - Simple method to obtain accurate pretilt angles for thin twisted nematic liquid-crystal... 22956618 - The effect of monocyte chemoattractant protein-1/cc chemokine ligand 2 on aqueous humor... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-3-27 |
Journal Detail:
|
Title: Molecular genetics and genomics : MGG Volume: - ISSN: 1617-4623 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-3-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101093320 Medline TA: Mol Genet Genomics Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Institut de Biologie et de Technologies de Saclay, Service de Biologie Intégrative et de Génétique Moléculaire, Bât. 144, CEA/Saclay, 91191, Gif-sur-Yvette Cedex, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Arm site independence of coliphage HK022 integrase in human cells.
Next Document: Genetic analysis of Down syndrome-associated heart defects in mice.