Document Detail

Giant cell tumor of bone: analysis of proliferative cells by double-labeling immunohistochemistry with anti-proliferating cell nuclear antigen antibody and culture procedure.
MedLine Citation:
PMID:  7914219     Owner:  NLM     Status:  MEDLINE    
Giant cell tumor of bone (GCT) consists of multinucleated giant cells, fibroblast-like cells, and macrophage-like cells, but what constitutes the principal neoplastic cells and their histogenesis remains controversial. Proliferating cells in GCT have been immunophenotypically characterized with the cell cycle-related antigen proliferating cell nuclear antigen (PCNA). In seven cases, PCNA monoclonal antibody was applied by double-labeling immunohistochemical methods together with other cell-specific monoclonal antibodies. Many proliferating PCNA-positive cells were found in the vimentin-positive fibroblast-like cells, while almost all macrophage-like cells were negative for PCNA. The study of cultured cells showed that the fibroblast-like spindle-shaped cells were a neoplastic element. In addition, osteoclast-like cells were detected in co-culture systems of cells cultured from GCT with spleen cells. These studies support the concept that GCT is seen as a neoplasm of fibroblast-like cells with a strong reactive infiltration of cells from the mononuclear phagocyte system.
Y Abe; K Yonemura; K Nishida; K Takagi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nihon Seikeigeka Gakkai zasshi     Volume:  68     ISSN:  0021-5325     ISO Abbreviation:  Nippon Seikeigeka Gakkai Zasshi     Publication Date:  1994 May 
Date Detail:
Created Date:  1994-09-06     Completed Date:  1994-09-06     Revised Date:  2011-07-27    
Medline Journal Info:
Nlm Unique ID:  0413716     Medline TA:  Nihon Seikeigeka Gakkai Zasshi     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  407-14     Citation Subset:  IM    
Department of Orthopaedic Surgery, Kumamoto University School of Medicine.
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MeSH Terms
Antibodies, Monoclonal
Bone Neoplasms / pathology*
Cell Division
Giant Cell Tumor of Bone / pathology*
Nuclear Proteins / analysis*
Proliferating Cell Nuclear Antigen
Tumor Cells, Cultured
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Nuclear Proteins; 0/Proliferating Cell Nuclear Antigen

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