Document Detail


The giant danio (D. aequipinnatus) as a model of cardiac remodeling and regeneration.
MedLine Citation:
PMID:  22095914     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The paucity of mammalian adult cardiac myocytes (CM) proliferation following myocardial infarction (MI) and the remodeling of the necrotic tissue that ensues, result in non-regenerative repair. In contrast, zebrafish (ZF) can regenerate after an apical resection or cryoinjury of the heart. There is considerable interest in models where regeneration proceeds in the presence of necrotic tissue. We have developed and characterized a cautery injury model in the giant danio (GD), a species closely related to ZF, where necrotic tissue remains part of the ventricle, yet regeneration occurs. By light and transmission electron microscopy (TEM), we have documented four temporally overlapping processes: (1) a robust inflammatory response analogous to that observed in MI, (2) concomitant proliferation of epicardial cells leading to wound closure, (3) resorption of necrotic tissue and its replacement by granulation tissue, and (4) regeneration of the myocardial tissue driven by 5-EDU and [(3) H]thymidine incorporating CMs. In conclusion, our data suggest that the GD possesses robust repair mechanisms in the ventricle and can serve as an important model of cardiac inflammation, remodeling and regeneration.
Authors:
Pascal J Lafontant; Alan R Burns; Jamie A Grivas; Mary A Lesch; Tanmoy D Lala; Sean P Reuter; Loren J Field; Tyler D Frounfelter
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-11-18
Journal Detail:
Title:  Anatomical record (Hoboken, N.J. : 2007)     Volume:  295     ISSN:  1932-8494     ISO Abbreviation:  Anat Rec (Hoboken)     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-12     Completed Date:  2012-05-01     Revised Date:  2013-09-17    
Medline Journal Info:
Nlm Unique ID:  101292775     Medline TA:  Anat Rec (Hoboken)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  234-48     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Affiliation:
DePauw University, Department of Biology, Greencastle, Indiana, USA. pascallafontant@depauw.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Proliferation
Deoxyuridine / analogs & derivatives,  metabolism
Disease Models, Animal
Granulation Tissue / pathology
Inflammation / pathology
Myocytes, Cardiac / metabolism,  pathology*,  ultrastructure
Necrosis
Neovascularization, Pathologic / metabolism,  pathology
Pericardium / pathology
Regeneration / physiology*
Thymidine / metabolism
Ventricular Remodeling / physiology*
Wound Healing / physiology
Zebrafish / physiology*
Grant Support
ID/Acronym/Agency:
EY007551/EY/NEI NIH HHS; EY017120/EY/NEI NIH HHS; P01 HL085098-05/HL/NHLBI NIH HHS; P30 EY007551-25/EY/NEI NIH HHS; R01 EY017120/EY/NEI NIH HHS; R01 HL109205/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
50-89-5/Thymidine; 61135-33-9/5-ethynyl-2'-deoxyuridine; 951-78-0/Deoxyuridine
Comments/Corrections

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