| Ghrelin treatment protects lactotrophs from apoptosis in the pituitary of diabetic rats. | |
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MedLine Citation:
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PMID: 19540304 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Poorly controlled diabetes is associated with hormonal imbalances, including decreased prolactin production partially due to increased lactotroph apoptosis. In addition to its metabolic actions, ghrelin inhibits apoptosis in several cell types. Thus, we analyzed ghrelin's effects on diabetes-induced pituitary cell death and hormonal changes. Six weeks after onset of diabetes in male Wistar rats (streptozotocin 70 mg/kg), minipumps infusing saline or 24 nmol ghrelin/day were implanted (jugular). Rats were killed two weeks later. Ghrelin did not modify body weight or serum glucose, leptin or adiponectin, but increased total ghrelin (P<0.05), IGF-I (P<0.01) and prolactin (P<0.01) levels. Ghrelin decreased cell death, iNOS and active caspase-8 (P<0.05) and increased prolactin (P<0.05), Bcl-2 (P<0.01) and Hsp70 (P<0.05) content in the pituitary. In conclusion, ghrelin prevents diabetes-induced death of lactotrophs, decreasing caspase-8 activation and iNOS content and increasing anti-apoptotic pathways such as pituitary Bcl-2 and Hsp70 and serum IGF-I concentrations. |
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Authors:
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M Granado; J A Chowen; C García-Cáceres; A Delgado-Rubín; V Barrios; E Castillero; J Argente; L M Frago |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-18 |
Journal Detail:
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Title: Molecular and cellular endocrinology Volume: 309 ISSN: 1872-8057 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-08-03 Completed Date: 2009-10-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: Ireland |
Other Details:
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Languages: eng Pagination: 67-75 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, Universidad Autónoma de Madrid, Madrid, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adiponectin
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blood Animals Apoptosis / drug effects* Biological Markers / metabolism Body Weight / drug effects Caspases / metabolism Cytoprotection / drug effects* Diabetes Mellitus, Experimental / blood, pathology* Ghrelin / blood, pharmacology* HSP70 Heat-Shock Proteins / metabolism In Situ Nick-End Labeling Insulin / blood Insulin-Like Growth Factor I / metabolism Lactotrophs / cytology*, drug effects*, enzymology Leptin / blood Nitric Oxide Synthase Type II / metabolism Prolactin / metabolism Proto-Oncogene Proteins c-bcl-2 / metabolism Rats Weight Gain / drug effects X-Linked Inhibitor of Apoptosis Protein / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Adiponectin; 0/Biological Markers; 0/Ghrelin; 0/HSP70 Heat-Shock Proteins; 0/Leptin; 0/Proto-Oncogene Proteins c-bcl-2; 0/X-Linked Inhibitor of Apoptosis Protein; 11061-68-0/Insulin; 67763-96-6/Insulin-Like Growth Factor I; 9002-62-4/Prolactin; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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