Document Detail

Ghrelin localization in the medulla of rat and human adrenal gland and in pheochromocytomas.
MedLine Citation:
PMID:  17952858     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Ghrelin is predominantly produced by neuroendocrine cells of stomach and has been expressed in several normal and tumour endocrine tissues. It has been reported that the localization of ghrelin is exclusively in the cortex of human and rat adrenal gland and in adrenocortical tumours. This prompted us to analyze the expression of this peptide in medulla of human and rat adrenal glands and in human pheochromocytomas.
DESIGN AND METHODS: Analysis of ghrelin mRNA expression in rat adrenal gland was conducted by means of semi-quantitative RT-PCR. Ghrelin localization was studied in medulla of human and rat adrenal gland by immunohistochemistry. In addition, we have carried out a double immunofluorescence with chromogranin A to determine the specific cell type expressing ghrelin immunoreactivity. Ghrelin expression was also analyzed in five cases of pheochromocytoma by immunohistochemistry. Finally, Western blotting analysis was performed with goat ghrelin antibody in the cortex and in the medulla of rat adrenal gland.
RESULTS: RT-PCR demonstrated expression of ghrelin mRNA in rat adrenal gland. We also detected ghrelin expression in virtually all rat pheochromocytes by immunohistochemistry and double immunofluorescence. Furthermore, we showed ghrelin immunoreactivity in the medulla of human adrenal gland and in pheochromocytomas. By Western blotting, we found the expression of ghrelin precursor, proghrelin and mature ghrelin in the medulla of rat adrenals. However, the cortex of rat adrenal gland only expressed ghrelin precursor.
CONCLUSIONS: Our study is the first to demonstrate a medullar expression of ghrelin in human and rat adrenal gland; we also showed ghrelin expression in pheochromocytomas.
K Raghay; T García-Caballero; S Bravo; C V Alvarez; R González; C Diéguez; A Beiras; M Fraga; R Gallego
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histology and histopathology     Volume:  23     ISSN:  1699-5848     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-10-22     Completed Date:  2007-11-07     Revised Date:  2011-10-19    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  57-65     Citation Subset:  IM    
Department of Morphological Sciences, Faculty of Medicine-University Clinical Hospital, University of Santiago de Compostela, Santiago de Compostela, Spain.
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MeSH Terms
Adrenal Cortex / metabolism,  pathology
Adrenal Gland Neoplasms / metabolism*,  pathology
Adrenal Medulla / metabolism*,  pathology
Middle Aged
Peptide Hormones / genetics,  metabolism*
Pheochromocytoma / metabolism*,  pathology
RNA, Messenger / metabolism
Rats, Sprague-Dawley
Receptors, G-Protein-Coupled / genetics,  metabolism
Receptors, Ghrelin
Reg. No./Substance:
0/Ghrelin; 0/Peptide Hormones; 0/RNA, Messenger; 0/Receptors, G-Protein-Coupled; 0/Receptors, Ghrelin

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