| Ghrelin Protects against the Detrimental Consequences of Porphyromonas gingivalis-Induced Akt Inactivation through S-Nitrosylation on Salivary Mucin Synthesis. | |
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MedLine Citation:
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PMID: 21603133 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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Disturbances in nitric oxide synthase isozyme system and the impairment in salivary mucin synthesis are well-recognized features associated with oral mucosal inflammatory responses to periodontopathic bacterium, P. gingivalis. In this study, using rat sublingual gland acinar cells, we report that P. gingivalis LPS-induced impairment in mucin synthesis and associated suppression in Akt kinase activity were accompanied by a decrease in constitutive nitric oxide synthase (cNOS) activity and an induction in inducible nitric oxide synthase (iNOS) expression. The LPS effect on Akt inactivation was manifested in the kinase S-nitrosylation and a decrease in its phosphorylation at Ser(473). Further, we demonstrate that a peptide hormone, ghrelin, countered the LPS-induced impairment in mucin synthesis. This effect of ghrelin was reflected in the suppression of iNOS and the increase in Akt activation, associated with the loss in S-nitrosylation and the increase in phosphorylation, as well as cNOS activation through phosphorylation. Our findings suggest that induction in iNOS expression by P. gingivalis-LPS leads to Akt kinase inactivation through S-nitrosylation that detrimentally impacts cNOS activation through phosphorylation as well as mucin synthesis. We also show that the countering effect of ghrelin on P. gingivalis-induced impairment in mucin synthesis is associated with Akt activation through phosphorylation. |
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Authors:
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Bronislaw L Slomiany; Amalia Slomiany |
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Publication Detail:
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Type: Journal Article Date: 2011-04-06 |
Journal Detail:
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Title: International journal of inflammation Volume: 2011 ISSN: 2042-0099 ISO Abbreviation: Int J Inflam Publication Date: 2011 |
Date Detail:
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Created Date: 2011-05-23 Completed Date: 2011-07-14 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 101538188 Medline TA: Int J Inflam Country: England |
Other Details:
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Languages: eng Pagination: 807279 Citation Subset: - |
Affiliation:
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Research Center, University of Medicine and Dentistry of New Jersey, P.O. Box 1709, Newark, NJ 07103-2400, USA. |
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