| Ghrelin Protects H9c2 Cardiomyocytes from Angiotensin II-induced Apoptosis Through the Endoplasmic Reticulum Stress Pathway. | |
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MedLine Citation:
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PMID: 22269847 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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ABSTRACT: Ghrelin, a gastric hormone, exerts cardio-protective function by increasing myocardial contractility and vasodilation. The previous studies have reported that angiotensin II (Ang II) production increased in heart failure, which can induce cardiomyocyte apoptosis. In the present study, we investigated the effect of ghrelin on Ang II-induced H9c2 cardiomyocyte apoptosis. The results showed that Ang II inhibited H9c2 cell viability, which was blocked by ghrelin. By annexin V-propidium iodide dual staining and TUNEL analysis, we found that Ang II induced H9c2 cell apoptosis, while co-incubation of ghrelin with Ang II significantly reduced H9c2 cell apoptosis induced by Ang II. Simultaneously, the results revealed that ghrelin regulated the Ang II-induced imbalance of Bax and Bcl-2 expression and reduced Ang II-induced caspase-3 expression. Moreover, mRNA expressions of endoplasmic reticulum stress-related molecules GRP78, caspase-12 and CHOP were significantly upregulated by Ang II. However, their expressions were significantly inhibited by ghrelin. In addition, we found that ghrelin markedly inhibited Ang II-induced AT1 receptor expression. These data suggest that ghrelin may play an antagonistic role in Ang II-induced cardiomyocyte apoptosis via decreasing AT1 receptor expression and inhibiting activation of endoplasmic reticulum stress pathway. |
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Authors:
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Chunyan Yang; Yinan Wang; Haiyan Liu; Nan Li; Yang Sun; Liu Zhonghui; Yang Ping |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-20 |
Journal Detail:
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Title: Journal of cardiovascular pharmacology Volume: - ISSN: 1533-4023 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7902492 Medline TA: J Cardiovasc Pharmacol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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*Department of cardiology, China-Japan Union Hospital, Jilin University, Changchun 130033, China †Department of Immunology §Department of Anatomy, Norman Bethune college of Medicine, Jilin University, Changchun 130021, China. |
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