| Gestational profile of matrix metalloproteinases in rat uterine artery. | |
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MedLine Citation:
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PMID: 12771236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mechanisms underlying structural reorganization of the uterine artery in pregnancy remain largely unknown. Matrix metalloproteinases (MMPs) which are involved in degradation of vascular wall matrix are likely to play a key role. In this investigation of rat uterine artery, key MMPs and the specific tissue inhibitors of MMPs (TIMPs) together with three housekeeping genes were studied before, during and after pregnancy, using real time PCR. Data were analysed by partial least squares analysis as well as by conventional univariate methods. Each gene studied [MMP-2, MMP-3, MMP-7, MMP-9, MMP-12, MMP-13, membrane-type 1 (MT1)-MMP, TIMP-1, TIMP-2, GAPDH, cyclophilin and beta-actin] increased in late pregnancy (day 21). MMP-2, MT1MMP, MMP-3 and TIMP-1 transcripts were also elevated at day 7. TIMP-1 and MMP-3 mRNA expression returned to virgin control values in the post-partum, whereas others remained elevated or increased further (MMP-9, MMP-13). Gelatin zymography showed maximum elevation of MMP-2 at day 21. A novel 43-45 kDa gelatinolytic doublet was observed which increased in density with gestation and may represent an active MMP-2 fragment. Together, these data strongly suggest that MMPs and TIMPs are likely to play an important role in remodelling uterine arteries in rat pregnancy and may represent means by which vasodilatation is maintained in later pregnancy. Continued elevated levels of some MMPs post-partum may contribute to vessel regression and return to a non-pregnant physiological state. |
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Authors:
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B A Kelly; B C Bond; L Poston |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular human reproduction Volume: 9 ISSN: 1360-9947 ISO Abbreviation: Mol. Hum. Reprod. Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-05-28 Completed Date: 2004-05-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9513710 Medline TA: Mol Hum Reprod Country: England |
Other Details:
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Languages: eng Pagination: 351-8 Citation Subset: IM |
Affiliation:
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Maternal and Fetal Research Unit, Department of Womens Health, Guy's, King's and St Thomas' School of Medicine, 10th Floor St Thomas' Hospital, Lambeth Palace Road, London SE1 7EH, UK. brenda.a.kelly@kcl.ac.uk |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arteries / enzymology* Female Matrix Metalloproteinases / antagonists & inhibitors, genetics, metabolism* Pregnancy Rats Tissue Inhibitor of Metalloproteinases / genetics, metabolism Uterus / blood supply* |
| Chemical | |
Reg. No./Substance:
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0/Tissue Inhibitor of Metalloproteinases; EC 3.4.24.-/Matrix Metalloproteinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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