| Gestational low protein diet in the rat mediates Igf2 gene expression in male offspring via altered hepatic DNA methylation. | |
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MedLine Citation:
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PMID: 20671425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Biological responses to environmental stress, including nutrient limitation are mediated in part by epigenetic modifications including DNA methylation. Insulin-like growth factor II (Igf2) and H19 are subject to epigenetic modifications leading to genomic imprinting. The present study was designed to test the effect of maternal low protein diet on the Igf2/H19 locus in offspring. Pregnant Sprague-Dawley rats were fed diets containing 180 g/kg casein (control) or 90 g/kg (LP) casein with either 1 mg/kg (LP) or 3 mg/kg folic acid (LPF). LP diet increased Igf2 and H19 gene expression in the liver of day 0 male offspring and the addition of folic acid reduced the mRNA level in LPF rats to that of the control group. DNA methylation in Imprinting Control Region (ICR) of Igf2/H19 locus increased significantly following maternal LP diet but rats fed the LPF diet did not exhibit the hypermethylation. The Differential Methylation Region 2 (DMR2) did not show any change in methylation in either LP or LPF rats. The expression of Dnmt1 and Dnmt3a, the members of DNA methyltransferase family, and methyl CpG-binding domain 2 (Mbd2) was significantly increased following the maternal LP diet but did not differ between the control and LPF group. There is a strong correlation between methylation of ICR with the expression of Igf2 and H19. These results suggested that maternal exposure to a low protein diet and folic acid during gestation alters gene expression of Igf2 and H19 in the liver by regulating the DNA methylation of these genes. The DNA methyltransferase machinery may be involved into the programming of imprinted genes through the imprinted control region. |
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Authors:
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Li Gong; Yuan-Xiang Pan; Hong Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-01 |
Journal Detail:
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Title: Epigenetics : official journal of the DNA Methylation Society Volume: 5 ISSN: 1559-2308 ISO Abbreviation: Epigenetics Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2011-04-05 Completed Date: 2011-06-28 Revised Date: 2011-10-07 |
Medline Journal Info:
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Nlm Unique ID: 101265293 Medline TA: Epigenetics Country: United States |
Other Details:
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Languages: eng Pagination: 619-26 Citation Subset: IM |
Affiliation:
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Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn DNA (Cytosine-5-)-Methyltransferase / genetics DNA Methylation* / drug effects DNA-Binding Proteins / genetics Diet, Protein-Restricted / adverse effects* Female Folic Acid / administration & dosage Gene Expression / drug effects Genomic Imprinting Insulin-Like Growth Factor II / genetics* Liver / drug effects, metabolism* Male Maternal-Fetal Exchange Pregnancy RNA, Untranslated / genetics Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/H19 long non-coding RNA; 0/MBD2 protein; 0/RNA, Untranslated; 59-30-3/Folic Acid; 67763-97-7/Insulin-Like Growth Factor II; EC 2.1.1.37/DNA (Cytosine-5-)-Methyltransferase; EC 2.1.1.37/DNA (cytosine-5-)-methyltransferase 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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