| Gestational changes in endothelium-dependent vasorelaxation in human umbilical artery. | |
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MedLine Citation:
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PMID: 8296828 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: We intended to clarify whether (1) the amount of endothelium-derived vasorelaxing factor or nitric oxide released by histamine is sufficient to cause relaxation in human umbilical artery at different stages of gestation and (2) the functional role of endothelium-derived relaxing factor changes with the progress of gestation. STUDY DESIGN: By means of a bioassay cascade technique with very thin muscle strips, which allows rapid diffusional access of applied drugs (of the order of a few seconds), contractile properties were examined. RESULTS: At 18 to 22 weeks of gestation histamine produced only a minor contraction, but as gestation progressed the contractile responses increased. L-NG-nitro-arginine greatly enhanced the histamine-induced contractions. Histamine produced a concentration-dependent relaxation during the maintained contraction induced by 39 mmol/L K+ in tissues from 18 to 22 weeks or 30 to 32 weeks but not 38 to 41 weeks of gestation. Thus the concentration-relaxation relationship for histamine showed decreased sensitivity during gestation. The histamine-induced relaxation was enhanced by superoxide dismutase and completely blocked by L-NG-nitro-arginine or mepyramine (H1 antagonist). The concentration-relaxation relationship for the action of glycerol trinitrate relaxation decreased as gestation progressed. In a bioassay cascade an endothelium-intact umbilical artery from 18 to 22 or 38 to 41 weeks of gestation was used as a "donor" and L-NG-nitro-arginine-treated umbilical artery from 18 to 22 and 38 to 41 weeks as a "detector." In the presence of histamine the perfusate that had passed through a donor from 18 to 22 weeks (but not 38 to 41 weeks) attenuated the high K(+)-induced contraction in the detector only from 18 to 22 weeks. CONCLUSION: These results suggest that the amount of endothelium-derived relaxing factor and the sensitivity of smooth muscle to endothelium-derived relaxing factor decreased with the progress of gestation. |
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Authors:
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H Izumi; R E Garfield; Y Makino; K Shirakawa; T Itoh |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of obstetrics and gynecology Volume: 170 ISSN: 0002-9378 ISO Abbreviation: Am. J. Obstet. Gynecol. Publication Date: 1994 Jan |
Date Detail:
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Created Date: 1994-03-01 Completed Date: 1994-03-01 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370476 Medline TA: Am J Obstet Gynecol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 236-45 Citation Subset: AIM; IM |
Affiliation:
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Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston 77551. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arginine
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analogs & derivatives,
pharmacology Biological Assay Dose-Response Relationship, Drug Endothelium, Vascular / metabolism, secretion Female Histamine / pharmacology, physiology Humans Nitric Oxide / metabolism, physiology*, secretion Nitroarginine Nitroglycerin / pharmacology Potassium / pharmacology Pregnancy / physiology* Pregnancy Trimester, Second Pregnancy Trimester, Third Pyrilamine / pharmacology Superoxide Dismutase / pharmacology Umbilical Arteries / physiology* Vasodilation / drug effects, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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ES04312/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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10102-43-9/Nitric Oxide; 2149-70-4/Nitroarginine; 51-45-6/Histamine; 55-63-0/Nitroglycerin; 74-79-3/Arginine; 7440-09-7/Potassium; 91-84-9/Pyrilamine; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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