Document Detail


Germline transmission of a novel rat embryonic stem cell line derived from transgenic rats.
MedLine Citation:
PMID:  22455749     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Germline-competent rat embryonic stem (ES) cell lines are important resources for the creation of mutant rat models using ES-cell-based gene targeting technology. The ability to isolate germline-competent ES cell lines from any rat strain, including genetically modified strains, would allow for more sophisticated genetic manipulations without extensive breeding. Sprague Dawley (SD) males carrying an enhanced green fluorescent protein (EGFP) transgene were used as the founder animals for the derivation of ES cell lines. A number of ES cell lines were established and subjected to rigorous quality control testing that included assessment of pluripotency factor expression, karyotype analysis, and pathogen/sterility testing. Two male ES cell lines, SD-Tg.EC1/Rrrc and SD-Tg.EC8/Rrrc, were injected into blastocysts recovered from a cross of Dark Agouti (DA) males with SD females. Resulting chimeric animals were bred with wild-type SD mates to verify the germline transmissibility of the ES cell lines by identifying pups carrying the ES cell line-derived EGFP transgene. While both ES cell lines gave rise to chimeric animals, only SD-Tg.EC1 was germline competent. This confirms the feasibility of deriving germline-competent ES cell lines from transgenic rat strains and provides a novel ES cell line with a stable green fluorescent protein (GFP) reporter for future genetic manipulations to create new rat models.
Authors:
Hongsheng Men; Beth A Bauer; Elizabeth C Bryda
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-05-04
Journal Detail:
Title:  Stem cells and development     Volume:  21     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-11     Completed Date:  2013-02-04     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2606-12     Citation Subset:  IM    
Affiliation:
Department of Veterinary Pathobiology, Rat Resource and Research Center, University of Missouri, Columbia, Missouri 65201, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques / methods
Cell Line*
Cell Shape
Chimera / genetics*
Embryo Transfer / methods
Embryo, Mammalian / cytology
Embryonic Stem Cells / cytology*
Female
Germ Cells / cytology*
Green Fluorescent Proteins / metabolism
Inheritance Patterns
Karyotype
Male
Octamer Transcription Factor-3 / genetics,  metabolism
Rats
Rats, Sprague-Dawley
Rats, Transgenic
Specific Pathogen-Free Organisms
Transcription Factors / metabolism
Transgenes*
Grant Support
ID/Acronym/Agency:
2P40 RR016939/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Nanog protein, rat; 0/Octamer Transcription Factor-3; 0/Transcription Factors; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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